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| Author(s): | Barcaccia, G.;
Mazzucato, A.;
Albertini, E.;
Zethof, J.;
Gerats, A.;
Pezzotti, M.;
Falcinelli, M. |
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| Author Affiliation: | Barcaccia, Albertini, Pezzotti, and Falcinelli: Istituto di Miglioramento Genetico Vegetale, Faculty of Agriculture, University of Perugia, Perugia, Italy; Mazzucato: Dipartimento di Agrobiologia e Agrochimica, University of Viterbo, Viterbo, Italy; and Zethof and Gerats: Laboratorium voor Genetica, Department of Genetics, University of Gent, Gent, Belgium |
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| Title: | Inheritance of parthenogenesis in Poa pratensis L.: Auxin test and AFLP linkage analyses support monogenic control |
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| Source: | Theoretical and Applied Genetics. Vol. 97, No. 1/2, 1998, p. 74-82. |
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| Publishing Information: | Berlin: Springer |
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| # of Pages: | 9 |
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| Keywords: | TIC Keywords: Parthenogenesis; Poa pratensis; Auxins; Amplification fragment length polymorphisms; Genes; Apomixis; Random amplified polymorphic DNA markers; Alleles; Breeding
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| Abstract/Contents: | "Gametophytic apomixis in Kentucky bluegrass (Poa pratensis L.) involves the parthenogenetic development of unreduced eggs from aposporic embryon sacs. Attempts to transfer the apomictic trait beyond natural sexual barriers require further elucidation of its inheritance. Controlled crosses were made between sexual clones and apomictic genotypes, and the parthenogenetic capacity of (poly)diploid hybrids was ascertained by the auxin test. A bulked segregant analysis with RAPD and AFLP markers was then used to identify a genetic linkage group related to the apomictic mode of reproduction. This approach enabled us to detect both an AFLP marker located 6.6 cM from the gene that putatively controls parthenogenesis and a 15.4-cM genomic window surrounding the target locus. A map of the P. pratensis chromosome region carrying the gene of interest was constructed using additional RAPD and AFLP markers that co-segregated with the parthenogenesis locus. Highly significant linkage between parthenogenesis and a number of AFLP markers that also appeared to belong to a tight linkage block strengthens the hypothesis of monogenic inheritance of this trait. If a single gene is assumed, apomictic polyploid types of P. pratensis would be simplex for a dominant allele that confers parthenogenesis, and this genetic model would be further supported by the bimodal distribution of the degree of parthenogenesis exhibited in the (poly)diploid progenies from sexual x apomictic matings. The molecular tagging of apomixis in P. pratensis is an essential step toward marker-assisted breeding and map-based cloning strategies aimed at investigating and manipulating its mode of reproduction." |
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| Language: | English |
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| References: | 38 |
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| Note: | Pictures, b/w
Tables
Figures
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Barcaccia, G., A. Mazzucato, E. Albertini, J. Zethof, A. Gerats, M. Pezzotti, et al. 1998. Inheritance of parthenogenesis in Poa pratensis L.: Auxin test and AFLP linkage analyses support monogenic control. Theor. Appl. Genet. 97(1/2):p. 74-82. |
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