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Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Torres, Kenneth C.;
Porche-Sorbet, Rhonda |
Author Affiliation: | Department of Horticulture, Louisiana State University, Baton Rouge, LA |
Title: | Isolation of Protoplasts From Selected Warm and Cool Season Turfgrasses |
Meeting Info.: | Held August 10-18, 1986; University of California, Davis |
Source: | HortScience. Vol. 21, No. 3, June 1986, p. 716. |
Publishing Information: | St. Joseph, MI : American Society for Horticultural Science |
Keywords: | TIC Keywords: Protoplasts; Warm season turfgrasses; Cool season turfgrasses
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Abstract/Contents: | "The use of protoplast isolation and somatic hybridization may benefit the turfgrass industry by enhancing breeding programs and allowing for the screening and selection of a large number of variant cell lines. Propoplasts have been isolated from callus on cell suspensions of Agrostis, Festuca, Cynodon, and Stenotaphrum. Various concentrations of mannitol were evaluated to determine the optimum osmotic level. Best results were noted when a concentration of 7-10% mannitol were used. The best enzymatic combination for protoplast release was achieved when 7% cellulase Type II (Sigma Chemical) was combined with 2% pectinase. Significant differences in propoplasts yield were noted between calli derived from old versus new cultures. The addition of 0.5 to 2.0% drieslase had no significant effect on protoplasts release. Protoplasts were subsequently cultured on a modified Murashige and Skoog culture medium." |
Language: | English |
References: | 0 |
Note: | As referenced in HortScience; Vol. 21:3; Section 2 or 2; Program and Abstracts; University of California, Davis; 22nd International Horticultural Congress; and 83rd Annual Meeting of the American Society for Horticultural Science. This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Torres, K. C., and R. Porche-Sorbet. 1986. Isolation of Protoplasts From Selected Warm and Cool Season Turfgrasses. HortScience. 21(3):p. 716. |
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