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| Web URL(s): | http://www.apsnet.org/publications/PlantDisease/BackIssues/Documents/1986Articles/PlantDisease70n11_1038.pdf
Last checked: 10/13/2015
Requires: PDF Reader |
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| Publication Type:
| Refereed |
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| Author(s): | Jeffers, S. N.;
Martin, S. B. |
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| Author Affiliation: | Department of Plant Pathology, New York State Agricultural Experiment Station, Cornell University, Geneva |
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| Title: | Comparison of Two Media Selective for Phytophthora and Pythium Species |
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| Source: | Plant Disease. Vol. 70, No. 11, November 1986, p. 1038-1043. |
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| Publishing Information: | St. Paul, Minn., American Phytopathological Society |
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| Keywords: | TIC Keywords: Phytophthora; Pythium
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| Abstract/Contents: | "The standard selective medium for isolating pythiaceous fungi, P10 VP (cornmeal agar amended with 10 mg/L pimaricin, 200 mg/L vancomycin, and 100 mg/L PCNB), was compared with a modified version of this medium, P5 ARP (primaricin reduced to 5 mg/L and vancomycin replaced by 250 mg/L ampicillin + 10 mg/L rifampicin). Hymexazol (50 mg/L) was added (i.e., P10 VPH and P5 ARPH) to suppress unwanted Pythium colonies for direct enumeration of Phytophthora from soil. P5 ARP(H), when compared with P10 VP(H), 1) detected more germinable propagules of P. cinnamomi, P. parasitica var. nicotianae, and Pythium ultimum from naturally infested soils, 2) suppressed contaminating bacteria much better in the test soil, 3) was equally effective in isolating P. megasperma and P. cactorum from apple twigs, 4) was much less expensive to prepare, and 5) supported mycelium growth of 42 isolates of Phytophthora (13 species) and 10 isolates of Pythium (six species) equally well. PCNB was beneficial for reducing or eliminating background mycoflora on soil dilution plates. Hymexazol added to P5 ARP greatly inhibited P. cactorum and drastically limited the radial growth of one isolate each of P. megasperma f. sp. medicaginis, P. megasperma f. sp. glycinea, and P. capsici. P5 ARP(H) is an improvement over P10 VP(H) as a multipurpose selective medium for isolating Phytophthora and Pythium species from plant tissue and soil." |
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| Language: | English |
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| References: | 31 |
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| Note: | Tables
Figures |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Jeffers, S. N., and S. B. Martin. 1986. Comparison of Two Media Selective for Phytophthora and Pythium Species. Plant Dis. 70(11):p. 1038-1043. |
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| Web URL(s):
http://www.apsnet.org/publications/PlantDisease/BackIssues/Documents/1986Articles/PlantDisease70n11_1038.pdf
Last checked: 10/13/2015
Requires: PDF Reader |
| 
MSU catalog number: SB 599 .P95 |
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