| |
| Publication Type:
| Refereed |
|---|
| Author(s): | Dumsday, J. L.;
Smith, K. F.;
Forster, J. W.;
Jones, E. S. |
|---|
| Author Affiliation: | Dumsday: Plant Biotechnology Centre, Agriculture Victoria, Department of Primary Industries, La Trobe University, Bundoora, Victoria; Smith: Pastoral and Veterinary Institute, Agriculture Victoria, Department of Natural Resources and Environment, Hamilton, Victoria, Cooperative Research Centre for Molecular Plant Breeding, Australia; Forster and Jones: Plant Biotechnology Centre, Agriculture Victoria, Department of Primary Industries, La Trobe University, Bundoora, Victoria, Cooperative Research Centre for Molecular Plant Breeding, Australia |
|---|
| Title: | SSR-based genetic linkage analysis of resistance to crown rust (Puccinia coronata f. sp. lolii) in perennial ryegrass (Lolium perenne) |
|---|
| Source: | Plant Pathology. Vol. 52, No. 5, October 2003, p. 628-637. |
|---|
| Publishing Information: | Oxford : Blackwell Scientific Publications |
|---|
| # of Pages: | 10 |
|---|
| Keywords: | TIC Keywords: Genes; Genetic resistance; Disease resistance; Puccinia coronata; Lolium perenne; Simple sequence repeats
|
|---|
| Abstract/Contents: | "Crown rust (caused by Puccinia coronata f. sp. lolii) is a serious foliar disease of the pasture and turfgrass perennial ryegrass (Lolium perenne). Previous genetic studies have detected both qualitative and quantitative resistance mechanisms, and interpretation of the genetic system is complicated by variation within the sexually reproducing pathogen. Resistant and susceptible parental genotypes of ryegrass were identified using a composite urediniospore population collected from three geographically distinct locations. A two-way pseudo-testcross mapping population was obtained as the F1 progeny of the pair-cross between ryegrass parental genotypes Vedette6 and Victorian9. Both parents showed intermediate resistance against a pathogen population collected in a single geographical zone (Hamilton, Victoria), but in the F1 population, significant variation for a range of resistance-associated characters was detected. Statistical analysis of phenotypic data suggested a major gene effect, hence bulked segregant analysis with map-assigned simple sequence repeat (SSR) markers was used to scan the genome. A marker showing strong association with resistance was assigned to linkage group (LG) 2 of perennial ryegrass. Analysis of 11 LG2 SSR markers defined an interval between loci xlpssrh03f03 and xlpssrk02e02 as containing the gene or genes (LpPc1) conferring crown rust resistance. Resistance gene determinants were inherited from both parents, with up to 80% of the total phenotypic variation explained by markers segregating from Vedette6 and up to 26% of the variation explained by markers segregating from Victorian9. The two contributions together resulted in an additive increase in effect, with fully resistant individuals requiring determinants from both parents. A conserved syntenic relationship was observed with linkage group B of Avena strigosa, which is the location of a cluster of resistance genes to the oat form of crown rust. The implications of this study for marker-assisted selection of disease resistance in perennial ryegrass are discussed." |
|---|
| Language: | English |
|---|
| References: | 39 |
|---|
| Note: | Tables
Graphs |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Dumsday, J. L., K. F. Smith, J. W. Forster, and E. S. Jones. 2003. SSR-based genetic linkage analysis of resistance to crown rust (Puccinia coronata f. sp. lolii) in perennial ryegrass (Lolium perenne). Plant Pathol. 52(5):p. 628-637. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=91764 |
| If there are problems with this record, send us feedback about record 91764. |
| Choices for finding the above item: |
|---|
| 
MSU catalog number: SB 599 .P96 |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
