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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2003.93.6.S1#page=33
Last checked: 10/16/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Harmon, P. F.;
Dunkle, L. D.;
Latin, R. |
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| Author Affiliation: | Harmon and Latin: Department of Botany and Plant Pathology; Dunkle: United States Department of Agriculture, Agriculture Research Service, Purdue University, West Lafayette, Indiana |
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| Title: | A rapid PCR-based method for the detection of Magnaporthe oryzae from infected perennial ryegrass |
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| Meeting Info.: | 2003 APS Annual Meeting, August 9-13, Charlotte, North Carolina |
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| Source: | Phytopathology. Vol. 93, No. 6, June 2003, p. S33. |
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| Publishing Information: | St. Paul, MN: American Phytopathological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Polymerase chain reaction; Disease identification; Pyricularia grisea; Gray leaf spot; Lolium perenne; Disease control; DNA
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| Abstract/Contents: | "Gray leaf spot caused by Magnapothe oryzae is a serious disease of perennial ryegrass. Because turf managers must select appropriate fungicides for remedial treatment, accurate and timely identification of the pathogen is essential for efficient and effective disease management. We developed a PCR-based method to detect M. oryzae in infected perennial ryegrass tissue. The method utilizes a commercially available kit that is used for isolation and amplification of plant DNA from leaf tissue. The kit protocol was modified and found to be reliable for the extraction of M. oryzae DNA from leaf lesions. Primers were designed to amplify a 687-bp fragment of the Pot2 transposon that is found in multiple copies in the genome of the pathogen. The protocol amplified amounts of purified DNA as low as 5 pg and consistently and specifically detected M. oryzae in single diseased leaf blades as well as in field samples of infected perennial ryegrass." |
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| Language: | English |
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| References: | 0 |
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| See Also: | See also full article in Plant Disease, 87(9) September 2003, p. 1072-1076, R=90720 R=90720 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Harmon, P. F., L. D. Dunkle, and R. Latin. 2003. A rapid PCR-based method for the detection of Magnaporthe oryzae from infected perennial ryegrass. Phytopathology. 93(6):p. S33. |
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| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2003.93.6.S1#page=33
Last checked: 10/16/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: SB 599 .P48 |
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