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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2003.93.6.S1#page=72
Last checked: 10/16/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Ragatz, R.;
Scheef, E. A.;
Jung, G. |
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| Author Affiliation: | Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin |
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| Title: | Rapid DNA extraction using single field collected scleotium for the identification of Typhula species and varieties |
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| Meeting Info.: | 2003 Annual Meeting, August 9-13, Charlotte, North Carolina |
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| Source: | Phytopathology. Vol. 93, No. 6, June 2003, p. S72. |
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| Publishing Information: | St. Paul, MN: American Phytopathological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: DNA; Disease identification; Pathogens; Typhula; Typhula incarnata; Typhula ishikariensis; Polymerase chain reaction; Typhula phacorrhiza
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| Abstract/Contents: | "Typhula fungi are a psychrophilic pathogen of grasses and cereals found throughout the Northern Hemesphere [Hemisphere]. Two species, T. incarnata and T. ishikariensi s [ishikariensis] are highly pathogenic in North America to turf grasses and some cereals during the winter months of the year. Combined they are the second most damaging turf disease in the Great Lakes region and fourth in the United States. PCR primers specific for Typhula incarnata, T. phacorrhiza, and T. ishikariensis var. ishikariensis, var. idahoensis, var. canadensis have been developed but the current molecular identification method takes several months to complete due to slow growth. However, we have modified a leaf-diversity procedure to create a fast (several hours) and accurate DNA extraction protocol for a single Typhula sclerotium. Coupled with the PCR primers, Typhula varieties and species can be easily identified." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Ragatz, R., E. A. Scheef, and G. Jung. 2003. Rapid DNA extraction using single field collected scleotium for the identification of Typhula species and varieties. Phytopathology. 93(6):p. S72. |
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| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2003.93.6.S1#page=72
Last checked: 10/16/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: SB 599 .P48 |
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