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Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2002.92.6.S1#page=17 Last checked: 10/16/2015 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Curley, J.;
Warnke, S.;
Barker, R.;
Leong, S.;
Jung, G. |
Author Affiliation: | Curley, Leong and Jung: Deptartment of Plant Pathology, University of Wisconsin, Madison, Wisconsin; and Warnke and Barker: USDA-ARS National Forage Seed Production Research Center, Oregon State University, Corvallis, Oregon |
Title: | Genetic variabilty and QTL mapping of resistance to gray leaf spot in a Lolium pseudo-testcross population |
Section: | 2002 APS annual meeting abstracts of presenations Other records with the "2002 APS annual meeting abstracts of presenations" Section
|
Meeting Info.: | APS Annual Meeting, July 27-31, 2002, Milwaukee, WI |
Source: | Phytopathology. Vol. 92, No. 6, June 2002, p. S17. |
Publishing Information: | St. Paul, MN: American Phytopathological Society |
# of Pages: | 1 |
Keywords: | TIC Keywords: Genetic variability; Gray leaf spot; Disease resistance; Lolium; Hybrids; Random amplified polymorphic DNA markers; Restriction fragment length polymorphism; Amplification fragment length polymorphisms; Fungal diseases; Lolium perenne; Pyricularia grisea; Mapping
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Abstract/Contents: | "Gray leaf spot (GLS) is a serious fungal disease on the important turf and forage species, perennial ryegrass (Lolium perenne) caused by the rice blast fungus Magnaporthe grisea. Early reports suggest little resistance is present in perrenial ryegrass cultivars. However, greenhouse inoculations in our lab using several rice and ryegrass isolates suggests some resistance is present, both in sixteen F1 clones derived from crosses among individual clones of cultivars, and in a subsample of progenies from an annual x perennial ryegrass (MFA x MFB) F2 pseudo-testcross population. These two groups of genotypes varied widely in resistance, as measured by lesion type and severity. The results were generally consistent, and the clones MFA and MFB were generally more tolerant than others. Also, the porgeny subsample, when scored by the most severe lesion present of the three replicates, appears to segregate in a 1:2:1 ratio, though this needs confirmation. Thus, both the MFA x MFB population and the F1 genotypes show potential for use in quantitative trait locus (QTL) mapping and resistance breeding through recurrent selection. This study will examine variabiltiy in resistance, select parents for a new F2 pseudo-testcross mapping population, and make crosses. Also, genetic linkage map construction will begin using AFLP and RAPD markers, and RFLP synteny anchor probes from cereal crops. A partial map has already been constructed for the existing MFA x MFB population, and the phenotypic segregation seen in the subsample will enable QTL analysis in this population. With this information, locations and effects of QTL for GLS resistance in both rice and ryegrass can be compared via comparative QTL mapping in two different populations, which can then lead to map-based cloning of novel resistance genes in both species." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Curley, J., S. Warnke, R. Barker, S. Leong, and G. Jung. 2002. Genetic variabilty and QTL mapping of resistance to gray leaf spot in a Lolium pseudo-testcross population. Phytopathology. 92(6):p. S17. |
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| Web URL(s): http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2002.92.6.S1#page=17 Last checked: 10/16/2015 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: SB 599 .P48 |
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