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Web URL(s): | https://turf.rutgers.edu/research/abstracts/symposium2004.pdf#page=40 Last checked: 02/06/2017 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Bonos, Stacy A.;
Abraham, Eleni;
Aa, Melissa;
Kubik, Christine;
Meyer, William A. |
Author Affiliation: | Department of Plant Biology and Pathology, Rutgers University |
Title: | Molecular identification of Texas x Kentucky bluegrass hybrids |
Section: | Poster Other records with the "Poster" Section
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Meeting Info.: | Cook College, Rutgers, NJ: January 15-16, 2004 |
Source: | Proceedings of the Thirteenth Annual Rutgers TurfgrassSymposium. 2004, p. 39. |
Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
# of Pages: | 1 |
Keywords: | TIC Keywords: Cultivar evaluation; Cultivar identification; Cultivar improvement; Drought resistance; Heat resistance; Molecular genetics; Poa pratensis X Poa arachnifera
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Abstract/Contents: | "Although Kentucky bluegrass (Poa pratensis L.) cultivars have been developed with good turfgrass quality, irrigation is often necessary to maintain quality during heat and drought stress conditions. Interspecific hybridization between Texas bluegrass (Poa ar achnifera Torr.), a native to Texas and Oklahoma, and Kentucky bluegrass is being utilized to incorporate genes for heat and drought tolerance into Kentucky bluegrass. The breeding technique involves an initial inter-specific hybridization between Texas and Kentucky bluegrass, followed by modified backcrossing to Kentucky bluegrass (recurrent parent). DNA markers, such as SCAR (sequence characterized amplified region) markers, can be used to differentiate species and identify inter-specific hybrids between these two species. The objective of this study was to develop specific SCAR markers for Kentucky bluegrass and Texas bluegrass and use them to identify F1 and BC generation hybrids between these two species. SCAR markers were developed by sequencing a single RAPD (Random Amplified Polymorphic DNA) band and designing primers to amplify the chosen band. Two SCAR primer pairs were designed to identify Kentucky bluegrass and Texas bluegrass. Eight Kentucky bluegrass cultivars, four Texas bluegrass plants and 12 hybrids from F1 through BC3 generations were analyzed with the SCAR primer pairs. The respective SCAR markers differentiated between Kentucky bluegrass and Texas bluegrass plants in all cases. All F1 and BC2 hybrids amplified both SCAR markers. Some BC3 hybrids had lost the Texas bluegrass SCAR marker indicating introgression on the Kentucky bluegrass DNA in this generation. This breeding strategy of crossing Kentucky bluegrasss with Texas bluegrass could expand the adaptation of Kentucky bluegrass through transition zone areas and into southern states where better heat and drought tolerance is needed for better performance." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Bonos, S. A., E. Abraham, M. Aa, C. Kubik, and W. A. Meyer. 2004. Molecular identification of Texas x Kentucky bluegrass hybrids. Proc. Annu. Rutgers Turfgrass Symp. p. 39. |
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| Web URL(s): https://turf.rutgers.edu/research/abstracts/symposium2004.pdf#page=40 Last checked: 02/06/2017 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: SB 433 .R88 |
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