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| Web URL(s): | https://turf.rutgers.edu/research/abstracts/symposium2004.pdf#page=49
Last checked: 02/06/2017
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Li, Huaijun Mike;
Rotter, David;
Belanger, Faith C. |
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| Author Affiliation: | Department of Plant Biology and Pathology, Rutgers University |
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| Title: | Identification of a disease-responsive gene present in creeping bentgrass but not in other Agrostis species |
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| Section: | Poster
Other records with the "Poster" Section
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| Meeting Info.: | Cook College, Rutgers, NJ: January 15-16, 2004 |
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| Source: | Proceedings of the Thirteenth Annual Rutgers TurfgrassSymposium. 2004, p. 48. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Agrostis; Agrostis stolonifera; Breeding improvement; Disease resistance; Dollar spot; Gene isolation; Genetic analysis; Sclerotinia homoeocarpa
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| Abstract/Contents: | "Creeping bentgrass (Agrostis stolonifera L.), an important turfgrass species, is highly susceptible to dollar spot disease caused by the fungus Sclerotinia homoeocarpa F. T. Bennett. Colonial bentgrass (A. capillaris L.), a closely related species, has good resistance. Several interspecific hybrids between creeping bentgrass and colonial bentgrass showed excellent dollar spot resistance in field tests (Belanger et al., 2004). In order to investigate the mechanism of dollar spot resistance in the interspecific hybrids, we are using suppression subtractive hybridization (SSH) to detect genes unique or overexpressed in either creeping bentgrass or an interspecific hybrid following fungal inoculation. RNA samples of a resistant hybrid (#15) and its susceptible creeping bentgrass parent were extracted from leaf tissues of dollar spot-inoculated plants. Two cDNA libraries were constructed using SSH to enrich for hybrid-specific clones and creeping bentgrass-specific clones. A disease-responsive gene was identified from two of the clones from the creeping bentgrass-specific library. The full-length sequence of the cDNA was obtained through RACE (rapid amplification of cDNA ends) PCR. The gene encoded a 319 amino acid protein of 34753 Daltons. The amino acid sequence of the protein was homologous to a wheat benzothiazol-induced protein, a wheat Hessian fly response protein, a maize beta-glucosidase aggregating factor, a barley jasmonate-regulated gene, and a wheat vernalization-related gene. CDART (conserved domain architecture retrieval tool) analysis revealed it had two conserved domains, a plant disease resistance response protein domain and a jacalin-like lectin domain. Northern blot analysis confirmed its expression was increased in creeping bentgrass upon dollar spot infection. No expression was detected in hybrid #15 or colonial bentgrass from either greenhouse or field samples. These results validated the effectiveness of the SSH method. Southern blot analysis indicated creeping bentgrass and another hybrid (#14) each had a single copy of this gene. Interestingly we didn't detect any homologs from related bentgrass species, such as colonial bentgrass, velvet bentgrass (A. canina), or redtop (A. gigantea). The apparent uniqueness of this gene to creeping bentgrass is very interesting regarding the evolution of creeping bentgrass." |
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| Language: | English |
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| References: | 1 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Li, H. M., D. Rotter, and F. C. Belanger. 2004. Identification of a disease-responsive gene present in creeping bentgrass but not in other Agrostis species. Proc. Annu. Rutgers Turfgrass Symp. p. 48. |
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| Web URL(s):
https://turf.rutgers.edu/research/abstracts/symposium2004.pdf#page=49
Last checked: 02/06/2017
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: SB 433 .R88 |
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