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Web URL(s): | https://turf.rutgers.edu/research/abstracts/symposium2004.pdf#page=50 Last checked: 02/06/2017 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Li, Huaijun Mike;
Bonos, Stacy;
Meyer, William;
Belanger, Faith C. |
Author Affiliation: | Department of Plant Biology and Pathology, Rutgers University |
Title: | Search for RAPD markers linked to dollar spot resistance in colonial x creeping bentgrass hybrids |
Section: | Poster Other records with the "Poster" Section
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Meeting Info.: | Cook College, Rutgers, NJ: January 15-16, 2004 |
Source: | Proceedings of the Thirteenth Annual Rutgers TurfgrassSymposium. 2004, p. 49. |
Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
# of Pages: | 1 |
Keywords: | TIC Keywords: Agrostis stolonifera; Agrostis tenuis; Breeding improvement; Disease resistance; Dollar spot; Interspecific hybridization; Random amplified polymorphic DNA markers; Sclerotinia homoeocarpa
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Abstract/Contents: | "Dollar spot, caused by Sclerotinia homoeocarpa F. T. Bennett, is among the most serious diseases of creeping bentgrass (Agrostis stolonifera L.), an outstanding cool season turfgrass species that is extensively used on golf courses. Currently, management of dollar spot relies heavily on the use of fungicides. Durable genetic resistance is much more desirable given the development of fungicide resistance and restrictions on fungicide usage. Colonial bentgrass (A. capillaris L.) a closely related species that is also occasionally used on golf courses, is recognized by breeders as having good resistance against dollar spot. Interspecific hybridization followed by backcrossing, which has been successfully used in breeding improved cultivars of numerous crop species, may be a useful approach to introduce disease resistance from colonial bentgrass into creeping bentgrass (Belanger et al., 2003; Belanger et al., 2004). In the summer of 2002, a dollar spot resistant colonial x creeping hybrid was crossed with a creeping bentgrass plant. In the spring of 2003, a population of 282 progeny individuals (four replicates of each individual) was planted into a field plot in a randomized complete block design. The field was inoculated with the dollar spot pathogen. The plants were rated throughout the summer for percent disease turf. We are using bulked segregant analysis and random amplified polymorphic DNA (RAPD) to identify DNA markers linked to the colonial bentgrass-derived genetic contribution to the dollar spot resistance in the progeny. PCR amplified DNA bands present only in the hybrid and absent from the creeping bentgrass parent were considered to be colonial bentgrass-derived markers. The markers were scored for presence or absence in the resistant and susceptible individuals. The markers highly associated with the resistant group will be converted to sequence-characterized amplified region (SCAR) markers. These DNA markers will be useful in future marker-assisted selection for dollar spot resistance." |
Language: | English |
References: | 2 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Li, H. M., S. Bonos, W. Meyer, and F. C. Belanger. 2004. Search for RAPD markers linked to dollar spot resistance in colonial x creeping bentgrass hybrids. Proc. Annu. Rutgers Turfgrass Symp. p. 49. |
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| Web URL(s): https://turf.rutgers.edu/research/abstracts/symposium2004.pdf#page=50 Last checked: 02/06/2017 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: SB 433 .R88 |
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