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| Web URL(s): | https://turf.rutgers.edu/research/abstracts/symposium2004.pdf#page=54
Last checked: 02/06/2017
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Rotter, David;
Li, Huaijun Mike;
Belanger, Faith C. |
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| Author Affiliation: | Department of Plant Biology and Pathology, Rutgers University |
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| Title: | Suppression subtractive hybridization to identify genes overexpressed in colonial x creeping bentgrass hybrids relative to their creeping bentgrass parent |
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| Section: | Poster
Other records with the "Poster" Section
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| Meeting Info.: | Cook College, Rutgers, NJ: January 15-16, 2004 |
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| Source: | Proceedings of the Thirteenth Annual Rutgers TurfgrassSymposium. 2004, p. 53. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Agrostis stolonifera; Agrostis tenuis; Disease resistance; Dollar spot; Gene expression; Gene isolation; Interspecific hybrids; Sclerotinia homoeocarpa; Suppression subtractive hybridization
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| Abstract/Contents: | "Creeping bentgrass (Agrostis stolonifera L.), an important turfgrass species, is highly susceptible to dollar spot disease caused by the fungus Sclerotinia homoeocarpa F.T. Bennett. Colonial bentgrass (A. capillaries L.) is a closely related species that generally has good dollar spot resistance. Interspecific hybrids between creeping and colonial bentgrass have been made and several hybrids exhibited excellent resistance to dollar spot (Belanger et al., 2004). The molecular basis for this resistace can be investigated by using suppression subtractive hybridization (SSH) to detect low copy number transcripts that are unique or overexpressed in either the resistant hybrids or the creeping bentgrass parent following fungal inoculation. SSH is a method that allows one to subtract cDNAs from two samples, such as different physiological states or tissues, and then isolate genes that are transcribed at higher levels in one sample relative to the other. A benefit of this technique is that low and high copy number transcripts are normalized with respect to each other allowing for a higher chance of isolating rare transcripts. Two subtraction libraries were created from creeping and colonial bentgrass cDNAs, one is enriched for genes overexpressed in the hybrid relative to the creeping parent, and the other is enriched for genes overexpressed in the creeping parent relative to the hybrid. 960 colonies from the colonial specific library and 480 colonies from the creeping specific library were screened for differential expression using reverse northern dot blot analysis. On average, 14% of the colonies screened from both libraries were differentially expressed. Further studies will utilize either Norhtern analysis or reverse transcriptase real time PCR to confirm the differential expression of these cDNAs. Plasmids from the differential expressed colonies were sequenced. To date, 45 plasmids for the creeping subtraction library and 85 plasmids from the hybrid subtraction library have been sequenced. The average insert length ranged from 150 to 500 base pairs with the average length of 400 bp. The SSH technique has a restriction enzyme step, therefore none of the resulting clones will be full-length. Most of the clones sequenced are homologous to genes related to stress resistance in other plants, and so may be relevant to the disease response of the bentgrass." |
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| Language: | English |
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| References: | 1 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Rotter, D., H. M. Li, and F. C. Belanger. 2004. Suppression subtractive hybridization to identify genes overexpressed in colonial x creeping bentgrass hybrids relative to their creeping bentgrass parent. Proc. Annu. Rutgers Turfgrass Symp. p. 53. |
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| Web URL(s):
https://turf.rutgers.edu/research/abstracts/symposium2004.pdf#page=54
Last checked: 02/06/2017
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: SB 433 .R88 |
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