Full TGIF Record # 93203
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Web URL(s):http://archive.lib.msu.edu/tic/ressum/2003/24.pdf
    Last checked: 01/23/2017
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Author(s):Qu, Rongda
Author Affiliation:North Carolina State University
Title:Hybrid bermudagrass improvement by genetic transformation
Section:Breeding, genetics, and physiology
Other records with the "Breeding, genetics, and physiology" Section
Source:2003 Turfgrass and Environmental Research Summary. 2003, p. 24.
# of Pages:1
Publishing Information:Far Hills, NJ: United States Golf Association
Keywords:TIC Keywords: Hybrid bermudagrasses; Genetic transformation; Calli; Transgenic turfgrasses; Cynodon dactylon; Cynodon transvaalensis
See Also:Other Reports from this USGA research project: 1998-18-126
Note:Pictures, color
USGA Summary Points:Using highly regenerable callus lines developed in our lab, we have developed a protocol to obtain transgenic common bermudagrass plants by the biolistic (gene gun) method using hygromycin B selection. Four such plants are confirmed by DNA hybridization and other analysis. By co-transformation, one of them showed GUS reporter gene activity and three are resistant to the herbicide Finale. Multiple transgene copies in transgenic plants are observed. Using similar conditions, we also have been able to obtain two transgenic common bermudagrass plants by Agrobacterium-mediated transformation, which is confirmed by DNA hybridization. The nematode-resistance gene was obtained from a collaborator and gene constructs have been made. By using both biolistic and Agrobacterium approaches to deliver this gene construct, ten hyg B-resistant calluses were obtained. One of them is regenerating.
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Qu, R. 2003. Hybrid bermudagrass improvement by genetic transformation. Turfgrass Environ. Res. Summ. p. 24.
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    Last checked: 01/23/2017
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MSU catalog number: SB 433 .A1 A6
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