| |
| Web URL(s): | http://www.bioone.org/bioone/?request=get-document&issn=0015-4040&volume=086&issue=04&page=0450
Last checked: 11/2005
Access conditions: Item is within a limited access website |
|---|
| Publication Type:
| Report |
|---|
| Author(s): | Meagher, R. L. Jr.;
Gallo-Meagher, M. |
|---|
| Author Affiliation: | Meagher: Center for Medical, Agricultural and Veterinary Entomology, Agricultural Research Service, U.S. Department of Agriculture, Gainseville, FL; and Gallo-Meagher: University of Florida, Agronomy Department and Plant Molecular and Cellular Program, Gainesville, FL |
|---|
| Title: | Indentifying host strains of fall armyworm (Lepidoptera: Noctuidae) in Florida using mitochondrial markers |
|---|
| Source: | Florida Entomologist. Vol. 86, No. 4, December 2003, p. 450-455. |
|---|
| Publishing Information: | Gainesville, Florida: Florida Entomological Society |
|---|
| # of Pages: | 6 |
|---|
| Keywords: | TIC Keywords: Spodoptera frugiperda; Mitochondria; Insect identification; DNA; Restriction fragment length polymorphism; Polymerase chain reaction
|
|---|
| Geographic Terms: | Florida |
|---|
| Abstract/Contents: | "Two molecular techniques were used to identify host strains of fall armyworm, Spodoptera frugiperda (J. E. Smith) from male moths captured in pheromone-baited traps in north-central and central Florida. Moths collected in 1998 were analyzed using direct detection of mitochondrial DNA (mtDNA) RFLPs generated from restriction endonuclease digestion of total DNA, while moths collected in 2000 and 2001 were analyzed using a mitochondrial cytochrome oxidase subunit I (COI) gene PCR-RFLP marker. Both techniques could distinguish between rice and corn strains moths, however, the COI PCR-RFLP marker was more robust as indicated by a time interval experiment that showed that moths held up to 15 days in a "bucket trap" could still be used for strain diagnosis. In a field study, our strategy gave results consistent with expectations. Rice strain moths were common in habitats with large areas of small grasses, corn strain moths were common in large areas planted to corn, and habitats with mixed large- and small-grass plantings contained both strains. Our methodology of combining pheromone traps with PCR-RFLP analysis will provide a valubable sampling system to determine the population ecology habits and stain isolating mechanisms of fall armyworm populations in numerous habitats, including overwintering areas of southern Florida." |
|---|
| Language: | English |
|---|
| References: | 28 |
|---|
| Note: | Tables
Abstract also appears in spanish
Pictures, b/w |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Meagher, R. L. Jr., and M. Gallo-Meagher. 2003. Indentifying host strains of fall armyworm (Lepidoptera: Noctuidae) in Florida using mitochondrial markers. FLA Entomol. 86(4):p. 450-455. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=93298 |
| If there are problems with this record, send us feedback about record 93298. |
| Choices for finding the above item: |
|---|
| Web URL(s):
http://www.bioone.org/bioone/?request=get-document&issn=0015-4040&volume=086&issue=04&page=0450
Last checked: 11/2005
Access conditions: Item is within a limited access website |
| 
MSU catalog number: QL 461 .F5 |
| Find from within TIC:
Digitally in TIC by record number. |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
