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| Web URL(s): | http://usgatero.msu.edu/v02/n21.pdf
Last checked: 3/2004
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Author(s): | Maqbool, S. B.;
Sticklen, M. B. |
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| Author Affiliation: | Maqbool: Research Associate Postdoc; and Sticklen: Professor, Cereal and Turfgrass Genetic Engineering Laboratory, Department of Crop and Soil Sciences, Michigan State University, East Lansing, Michigan |
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| Title: | Genetic engineering turfgrasses for pest resistance |
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| Source: | USGA Turfgrass and Environmental Research Online. Vol. 2, No. 21, November 2003, p. [1-15]. |
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| Publishing Information: | Far Hills, NJ: United States Golf Association, Green Section |
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| # of Pages: | 15 |
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| Keywords: | TIC Keywords: Pest resistance; Genetic transformation; Genetic markers; Agrostis stolonifera; Transgenic turfgrasses; Calli; Herbicide resistance; Glufosinate; Chitinase; Disease resistance
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| Trade Names: | Finale |
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| Abstract/Contents: | "Genetic engineering of crops is known to complement crop breeding for development of genotypes that are impossible to develop through breeding practices only. Michigan State University researchers: Developed an efficient cell and tissue culture turfgrass regeneration system that is the prerequisite for genetic transformation. Used embryogenic turfgrass callus (non-differentiated cell lines) to recover genetically altered turfgrass and developed a genetic engineering system for creeping bentgrass using a reporter (gus) blue gene to determine successful gene incorporation into plants. Successfully incorporated a gene (bar) for resistance to glufosinate (FinaleTM or bialaphos, a non-selective herbicide) and developed transgenic turfgrass plants resistant to this herbicide. Discovered that bialaphos also has fungicidal in addition to herbicidal properties. As a result, they were able to simultaneously control weeds and diseases caused by the pathogenic fungi Rhizoctonia solani (brown patch) and Sclerotinia homoeocarpa (dollar spot) by spraying the herbicide on transgenic creeping bentgrass expressing this gene. Cloned and characterized a full-length chitinase gene (hs2) that contains the necessary chitin-binding domain from a Dutch elm disease resistant American elm (Ulmus Americana). Constructed a mini-gene containing this chitinase gene and successfully inserted this chitinase gene into creeping bentgrass. Fungal bioassays utilizing chitinase expressing transgenic plants were conducted at laboratory and greenhouse levels in collaboration with Dr. Joseph Vargas. These bioassays showed that two out of five independently transgenic turfgrass lines were 3-5 fold resistant to R. solani." |
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| Language: | English |
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| References: | 22 |
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| See Also: | Other Reports from this USGA research project: 1994-06-078; 1998-07-115; 2001-02-200 |
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| Note: | Pictures, b/w
Figures
Tables
Summary as abstract |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Maqbool, S. B., and M. B. Sticklen. 2003. Genetic engineering turfgrasses for pest resistance. USGA Turfgrass Environ. Res. Online. 2(21):p. [1-15]. |
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| Web URL(s):
http://usgatero.msu.edu/v02/n21.pdf
Last checked: 3/2004
Requires: PDF Reader |
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MSU catalog number: SB 433 .A1 A65 [online] |
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