Full TGIF Record # 95099
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Web URL(s):http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS.2003.87.12.1426
    Last checked: 01/04/2008
    Requires: PDF Reader
Publication Type:
i
Refereed
Author(s):Avila-Adame, Cruz; Olaya, Gilberto; Köller, Wolfram
Author Affiliation:Avila-Adame and Köller: Department of Plant Pathology, Cornell University, New York State Agricultural Experiment Station, Geneva, New York; Olaya: Syngenta Crop Protection, Incorporated, Vero Beach, Florida
Title:Characterization of Colletotrichum graminicola isolates resistant to strobilurin-related QoI Fungicides
Source:Plant Disease. Vol. 87, No. 12, December 2003, p. 1426-1432.
Publishing Information:St. Paul, MN: American Phytopathological Society
# of Pages:7
Keywords:TIC Keywords: Colletotrichum graminicola; Fungicide resistance; QoI fungicides; Genes; DNA; Trifloxystrobin; Azoxystrobin; Pathogens
Abstract/Contents:"Isolates of Colletotrichum graminicola were collected from annual bluegrass or bent grass turf in Japan and the United States, and their sensitivities to QoI fungicides (QoIs) as well as their cytochrome ibr sequences were characterized. Five isolates sampled from turf treated repeatedly with azoxystrobin were highly QoI resistant under both in vivo and in vitro test conditions. The nucleotide sequences of a large cytochrome ibr gene segment involving the binding site of QoIs were fully homologous for all resistant isolates and contained the G143A target site mutation known to confer QoI resistance in other pathogens. QoI-sensitive isolates collected prior to treatments with QoIs were more diverse with regard to their cytochrome ibr gene sequences and their phenotype responses to QoIs. All wild-type isolates retained a glycine in position 143 of cytochrome ibr. Three of the four QoI-sensitive isolates were, in addition, distinguished by leucines in positions 95, 130, and 141, which were exchanged to threonine in all resistant but also in one of the sensitive isolates. In addition to a more pronounced divergence of cytochrome ibr sequences, the sensitive wild-type isolates also were diverse with regard to the induction of alternative respiration in reponse to QoI action, as indicated by comparisons of QoI sensitivities displayed in the absence or presence of the alternative oxidase inhibitor salicylhydroxamic acid. These different phenotype responses expressed under in vitro test conditions had no or only a slight impact on anthracnose control in protective applications of azoxystrobin. Isolate responses in vitro were very similar for trifloxystrobin, indicating cross-resistance among the class of QoIs. Our results imply that iC. graminicolar falls into the class of pathogens with a potential for rapid selection of highly QoI-resistant phenotypes. Frequent monitoring of population sensitivities will be required to determine the status of population responses toward practical QoI resistance."
Language:English
References:46
Note:Tables
Graphs
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Avila-Adame, C., G. Olaya, and W. Köller. 2003. Characterization of Colletotrichum graminicola isolates resistant to strobilurin-related QoI Fungicides. Plant Dis. 87(12):p. 1426-1432.
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Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS.2003.87.12.1426
    Last checked: 01/04/2008
    Requires: PDF Reader
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