Full TGIF Record # 96578
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Web URL(s):https://stri.lib.msu.edu/vol79-2003/pdf/sptri00079061.pdf
    Last checked: 02/01/2008
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Publication Type:
i
Refereed
Author(s):Salehi, H.; Khosh-Khui, M.
Author Affiliation:Department of Horticultural Science, College of Agriculture, Shiraz University, Shiraz, Iran
Title:Effects of acid treatment on in vitro seed culture of four turfgrass genera
Source:Journal of Turfgrass and Sports Surface Science. Vol. 79, 2003, p. 61-64.
Publishing Information:Bingley, West Yorkshire, UK: The Sports Turf Research Institute
# of Pages:4
Keywords:TIC Keywords: Seeds; Calli; Cynodon dactylon; Festuca rubra subsp. rubra; Lolium perenne; Poa pratensis
Cultivar Names:Shadow; Barball; Merion
Abstract/Contents:"Callus production from seed explants of turfgrasses is a primary stage for somatic embryogenesis or adventious shoot production commonly used in biotechnological methods including gene transformation. In some turfgrass genera low callus productionis the main problem in in vitro culture of seeds. In this study, the following treatments wee used: Cynodon dactylon [L.] Pers. with 50% sulphuric acid for 20 min.; Festuca rubra L. var. rubra 'Shadow' with 25% sulphuric acid for 15 min.; Lolium perenne L. 'Barball' with 50% sulphuric acid for 10 min. and Poa pratensis L. 'Merion' with 50% sulphuric acid for 15 min., all with controls. Interaction between these treatments and light or dark conditions was also investigated. Each treatment consisted of 4 replications, 100 seeds per replicate. After treatment abd surface sterilization, seeds were cultured on MS basal medium supplemented with 8 g l-1 agar-agar, 30 g l-1 sucrose and 40 μM 2,4-D for Cynodon and Poa, 150 μM 2,4-D for Lolium and 200 μM 2,4-D for Festuca. The number of cultured seeds producing callus was measured 4 weeks after the culturing. Results indicated that acid treatments improved both callus production percentage and rate (days to visible callus production), compared to control. Cynodon had the highest callus production percentage and thereafter were Poa, Lolium, and Festuca, respectively. Cynodon had the highest callus production rate and thereafter were Lolium, Poa, and Festuca, respectively. Cynodon had the best callus production in light and the other genera in dark conditions."
Language:English
References:14
Note:Tables
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Salehi, H., and M. Khosh-Khui. 2003. Effects of acid treatment on in vitro seed culture of four turfgrass genera. J. Turfgrass Sports Surf. Sci. 79:p. 61-64.
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Web URL(s):
https://stri.lib.msu.edu/vol79-2003/pdf/sptri00079061.pdf
    Last checked: 02/01/2008
    Requires: PDF Reader
    Notes: Available to TGIF and STRI users
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MSU catalog number: SB 433 .A1 S63 v. 78
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