Full TGIF Record # 98877
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Web URL(s):http://www.tandfonline.com/doi/pdf/10.1081/LCSS-200026796
    Last checked: 10/15/2015
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Author(s):Stiegler, J. C.; Bell, Gregory E.; Maness, N. O.
Author Affiliation:Stiegler: Payne County Extension Office, Stillwater, Oklahoma; Bell and Maness: Department of Horticulture and Landscape Architecture, Oklahoma State University, Stillwater, Oklahoma
Title:Comparison of acetone and N,N-dimethylformamide for pigment extraction in turfgrass
Source:Communications in Soil Science and Plant Analysis. Vol. 35, No. 13/14, 2004, p. 1801-1813.
# of Pages:13
Publishing Information:New York, NY: Marcel Dekker
Keywords:TIC Keywords: Poa annua; Chlorophyll; Agrostis stolonifera; Mowing; Laboratory methods; Fertilization; Tissue testing; Tissues
Abstract/Contents:"The extraction and isolation of plant pigments are critical parts of the procedures used for pigment analyses by plant scientists. Various organic solvents have been used to liberate chlorophylls and carotenoids, including xanthophylls, from plant tissue. Acetone extraction is the most common extractant, but other solvents, such as N,N-dimethylformamide (DMF), have been used successfully. The objectives of this study were to compare the repeatability of acetone and DMF extraction for turfgrass pigment analysis and to investigate which extraction method is more appropriate for sample preparation before analysis using high-performance liquid chromatography (HPLC). Leaf tissue was collected from three plots of a mixed sward of creeping bentgrass (Agrostis stolonifera L.) and annual bluegrass (Poa annua L.) that were differentially treated in the field with magnesium (Mg), iron (Fe), and/or a plant growth regulator to encourage a range of leaf pigment concentrations. Variation among tissue samples, pigment extraction, and the HPLC process was used to compare the precision of acetone with DMF extraction. Results indicated that both acetone and DMF extraction procedures exhibited acceptable variation, but DMF extraction was more consistent at each procedural level. Procedural levels included variation among tissue samples (coefficient of variation = 11.2 < 12.1), variation among HPLC measurements (coefficient of variation = 2.1 < 2.9), and variation among extracted samples (coefficient of variation = 4.8 < 12.7). The DMF extraction also yielded 84% more neoxanthin, 53% more violaxanthin, 47% more lutein, 104% more chlorophyll b, 145% more chlorophyll a, and 14% more Β-carotene than acetone. Overall, pigment extraction with DMF produced more quantitative and more reliable results than acetone extraction."
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Stiegler, J. C., G. E. Bell, and N. O. Maness. 2004. Comparison of acetone and N,N-dimethylformamide for pigment extraction in turfgrass. Commun. Soil. Sci. Plant Anal. 35(13/14):p. 1801-1813.
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    Last checked: 10/15/2015
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    Access conditions: Item is within a limited-access website
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