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| Web URL(s): | https://dl.sciencesocieties.org/publications/cs/articles/44/6/2168
Last checked: 11/16/2016
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https://dl.sciencesocieties.org/publications/cs/pdfs/44/6/2168
Last checked: 11/16/2016
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| Publication Type:
| Refereed |
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| Author(s): | Eaton, T. D.;
Curley, J.;
Williamson, R. C.;
Jung, G. |
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| Author Affiliation: | Eaton, Williamson: Department of Entomology ; Curley, Jung: Department of Plant Pathology, University of Wisconsin-Madison, Madison, Wisconsin |
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| Title: | Determination of the level of variation in polyploidy among Kentucky bluegrass cultivars by means of flow cytometry |
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| Section: | Turfgrass science
Other records with the "Turfgrass science" Section
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| Source: | Crop Science. Vol. 44, No. 6, November/December 2004, p. 2168-2174. |
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| Publishing Information: | Madison, WI: Crop Science Society of America |
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| # of Pages: | 7 |
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| Related Web URL: | https://dl.sciencesocieties.org/publications/cs/abstracts/44/6/2168
Last checked: 11/16/2016
Notes: Abstract only |
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| Keywords: | TIC Keywords: Polyploidy; Poa pratensis; Laser flow cytometry; Hybrids; DNA; Genetic markers
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| Abstract/Contents: | "Kentucky bluegrass (Poa pratensis L.) is an important cool-season grass species in the turfgrass and forage industries. Understanding the genetics of Kentucky bluegrass is useful in developing improved cultivars and hybrids. However, studying the genetics of Kentucky bluegrass can often be difficult because of the high variation in ploidy level that results from its facultative apomictic reproductive nature. Flow cytometry provides an easy and accurate method for assessing this variation by quantifying DNA content. The purpose of our study was to determine the level of variation in ploidy in Kentucky bluegrass by analyzing its DNA content using flow cytometry. In addition, DNA content was compared with genetic similarity derived from DNA marker data, and was also correlated with chromosome number. Twenty-two cultivars of Kentucky bluegrass were selected for the study by considering the range of variability in morphological traits and genetic distance derived from DNA marker data. We found that the DNA content of Kentucky bluegrass genotypes from the 22 cultivars ranged from 5.39 to 17.69 pg of DNA/2C and that a majority of the genotypes had a DNA content value in the range of 7 to 13 pg. A significant correlation between DNA content and chromosome number was detected. Euploid chromosome numbers (x = 7) with a range from the pentaploid (2n = 5x = 35) to the quindecaploid (2n = 15x = 105) were found along with aneuploid numbers. The results of this research could aid both breeders and researchers in studying the genetics of the species and in improving Kentucky bluegrass cultivars via intra- and interspecific hybridizations." |
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| Language: | English |
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| References: | 19 |
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| Note: | Pictures, b/w
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Eaton, T. D., J. Curley, R. C. Williamson, and G. Jung. 2004. Determination of the level of variation in polyploidy among Kentucky bluegrass cultivars by means of flow cytometry. Crop Sci. 44(6):p. 2168-2174. |
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| Web URL(s):
https://dl.sciencesocieties.org/publications/cs/articles/44/6/2168
Last checked: 11/16/2016
Access conditions: Item is within a limited-access website
https://dl.sciencesocieties.org/publications/cs/pdfs/44/6/2168
Last checked: 11/16/2016
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: SB 183 .C7 |
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