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| Web URL(s): | http://www.apsnet.org/publications/plantdisease/backissues/Documents/1987Articles/PlantDisease71n01_47.PDF
Last checked: 08/24/2010
Requires: PDF Reader |
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| Publication Type:
| Refereed |
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| Author(s): | Martin, Bruce |
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| Author Affiliation: | Assistnant Plant Pathologist, Department of Plant Pathology and Ecology, Connecticut Agricultural Experiment Station, New Haven, Connecticut |
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| Title: | Rapid tentative identification of Rhizoctonia spp. associated with diseased turfgrasses |
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| Source: | Plant Disease. Vol. 71, No. 1, January 1987, p. 47-49. |
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| Publishing Information: | St. Paul, Minn., American Phytopathological Society |
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| # of Pages: | 3 |
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| Keywords: | TIC Keywords: Identification keys; Rhizoctonia; Diseases; Flooding
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| Abstract/Contents: | "Fluorescence microscopy using the DNA-binding probe 4',6'-diamidino-2-phenylindole (DAPI) was used successfully to deterine number of nuclei per hyphal cell of Rhizoctonia spp. that cause patch diseases of turfgrasses. The procedure included growth of the hyphae from diseased tissue onto agar-coated microspoce slides followed by rapid fixation with 3% formaldehye. Samples were rinsed with distilled water and flooded with a 1-ppm solution of DAPI, then destained by flooding the sample with distilled water. Most of the water was then removed, a drop of glycerin was applied followed by a coverslip, and the saple was viewed using ultraviolet excitation with epiilumination and Neofluar objectives. Nuclei within isolates of R. solani, R. cerealis, R.zeae, and R. oryzae were clearly differentiated by the method. This method combines speed and reliability as a diagnostic tool." |
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| Language: | English |
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| References: | 13 |
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| Note: | Brief summary appears in The Journal of the Sports Turf Research Institute, June 1988, vol. 64, p. 198. [SB 433 .A1 S63]
Figures |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Martin, B. 1987. Rapid tentative identification of Rhizoctonia spp. associated with diseased turfgrasses. Plant Dis. 71(1):p. 47-49. |
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| Web URL(s):
http://www.apsnet.org/publications/plantdisease/backissues/Documents/1987Articles/PlantDisease71n01_47.PDF
Last checked: 08/24/2010
Requires: PDF Reader |
| 
MSU catalog number: SB 599 .P95 |
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