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| Author(s): | Cao, M.;
Sleper, D. A. |
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| Author Affiliation: | Department of Agronomy, University of Missouri-Columbia, Columbia, Missouri 65211, USA |
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| Title: | Use of genome-specific repetitive DNA sequences to monitor chromatin introgression from Festuca mairei into Lolium perenne |
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| Source: | Theoretical and Applied Genetics. Vol. 103, 2001, p. 248-253. |
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| Publishing Information: | Berlin: Springer |
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| # of Pages: | 6 |
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| Abstract/Contents: | "Repetitive DNA sequences contribute considerably to an understanding of the genomes of higher plants. Repetitive DNA sequences tend to be genome-specific due to the rate of amplification and extent of divergence. Two genome-specific probes from the genomic DNA library of Festuca arundinacea var. genuina Schreb. were selected and characterized. TF521 was found to be P genome-specific since it was able to hybridize with Festuca pratensis Huds. (PP) and Festuca arundinacea var. genuina (PPG1G1G2G2), but not, or only weakly, with tetraploid Festuca species. TF521 hybridized only with the diploid Festuca and not with the Lolium species (LL). TF436 was specific to tetraploid species of Festuca, such as F. arundinacea var. glaucescens Boiss. (G1G1G2G2) and Festuca mairei St. Yves (M1M1M2M2). By means of Southern hybridizations, TF436 was used to detect chomatin introgression of F. mairei in the progenies of the hybrid F. mairei x Lolium prenne L. Potential addition and translocation lines were identified in the BC1F1 derivatives of F. mairei x L. perenne. In situ hybridization was used to confirm the genetic identity of these lines. Sequence analysis indicated that TF436 and TF521 were two novel DNA sequences as no homologous sequences were found in Genebank." |
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| Language: | English |
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| References: | 32 |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Sleper, D. A. 2001. Use of genome-specific repetitive DNA sequences to monitor chromatin introgression from Festuca mairei into Lolium perenne. Theor. Appl. Genet. 103:p. 248-253. |
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