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| Publication Type:
| Refereed |
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| Author(s): | Sigler, William V.;
Nakatsu, Cindy H.;
Reicher, Zachary J.;
Turco Ronald F. |
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| Author Affiliation: | Department of Agronomy, Purdue University, West Lafayette, Indiana |
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| Title: | Fate of the biological control agent pseudomonas aureofaciens TX-1 after application to turfgrass |
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| Source: | Applied and Environmental Microbiology. Vol. 67, No. 8, August 2001, p. 3542-3548. |
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| Publishing Information: | Washington, D.C.: American Society for Microbiology |
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| # of Pages: | 7 |
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| Keywords: | TIC Keywords: Pseudomonas aureofaciens; Biological control; Biological disease control; Disease control; Soil microorganisms; Polymerase chain reaction; Sclerotinia homoeocarpa; Agrostis stolonifera; Biogation; Hybridization
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| Abstract/Contents: | "The fate and impact of Pseudomonas aureofaciens TX-1 following application as a biocontrol agent for fungi in turfgrass were studied. The organism was applied with a modified irrigation system by using a preparation containing 1x106 P. aureofaciens TX-1 CFU ml-1 about 100 times between May and August. We examined the impact of this repeated introduction of P. aureofaciens TX-1 (which is known to produce the antimicrobial compound pheazine-1-carboxylic acid) on the indigenous microbial community of the turfgrass system and on establishment of introduced bacteria in the soil system. A PCR primer-DNA hybridization probe combination was developed to accurately monitor the fate of P. aureofaciens TX-1 following application in irrigation water. To assess the impact of frequent P. aureofaciens TX-1 applications on the indigenous bacterial community, turfgrass canopy, thatch, and rhizosphere samples were obtained during the growing season from control and treated plots and subjected to DNA extraction procedures and denaturing gradient gel electrophoresis (DGGE). PCR amplification and hybridization of extracted DNA with the P. aureofaciens TX-1-specific primer-probe combination revealed that P. aureofaciens TX-1 not only became established in the rhizosphere and thatch but also was capable of overwintering. Separation of PCR-amplified partial 16S rRNA genes by DGGE showed that repeated application of P. aureofaciens TX-1 in irrigation water resulted in transient displacement of a leaf surface bacterial community member. There was no obvious alteration of any dominant members of the thatch and rhizosphere microbial communities." |
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| Language: | English |
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| References: | 42 |
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| Note: | Pictures, b/w
Figures
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Sigler, W. V., C. H. Nakatsu, Z. J. Reicher, and R. F. Turco. 2001. Fate of the biological control agent pseudomonas aureofaciens TX-1 after application to turfgrass. Appl. Environ. Microbiol. 67(8):p. 3542-3548. |
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