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| DOI: | 10.1016/S0929-1393(02)00088-4 |
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| Web URL(s): | http://www.sciencedirect.com/science/article/pii/S0929139302000884
Last checked: 05/29/2014
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Sigler, William V.;
Turco, Ronald F. |
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| Author Affiliation: | Sigler: Institute for Terrestrial Ecology, Grabenstrasse, Switzerland; Turco: Department of Agronomy, Purdue University, West Lafayette, IN |
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| Title: | The impact of chlorothalonil application on soil bacterial and fungal populations as assessed by denaturing gradient gel electrophoresis |
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| Source: | Applied Soil Ecology. Vol. 21, No. 2, September 2002, p. 107-118. |
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| Publishing Information: | [Amsterdam] : Elsevier Science |
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| # of Pages: | 12 |
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| Keywords: | TIC Keywords: Chlorothalonil; Comparisons; Fungicide application; Fungicide evaluation; Microbial fungicides; Microbiological soil analysis
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| Abstract/Contents: | "The impact of the fungicide chlorothalonil on dominant bacterial and fungal populations following application to turfgrass, forest, and agricultural soils was investigated. Chlorothalonil was applied to each soil at three rates, representing 0.2, 1 and 5 times the recommended label rate for turfgrass, and incubated for a 2-week period. Changes to the microbial community caused by the chlorothalonil application were assessed following DNA extraction, PCR-amplification using both bacteria domain- and fungal-specific primers, then separation with denaturing gradient gel electrophoresis (DGGE). Digitized DGGE images were used to determine two parameters: the number of bands per lane and the ShannonWiener index of diversity (H), both of which were used only for comparison of the different treatments, and not as true diversity measurements. Bands appearing to be either enhanced or inhibited as a result of the chlorothalonil treatment were excised and sequenced. Increased rates of chlorothalonil impacted eight bacterial populations (two inhibitions, four enhancements, and two non-specific responses) and four fungal populations (all inhibitions). Band number and H indicated an altered but not significantly different (P<0.05) bacterial and fungal community structure following chlorothalonil application. Sequencing of excised DGGE bands indicated an impact on several groups of bacteria (Cytophaga-Flavobacterium-Bacteroides, α-, β-, γ-, and δ-proteobacteria) and two fungal taxa (zygomycota and ascomycota). Although changes to the overall community structure of dominant species were non-significant, we conclude that following a single chlorothalonil application and a short incubation period, community changes including both enhancement and inhibition of a variety of dominant organisms can occur." |
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| Language: | English |
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| References: | 49 |
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| Note: | Correction appears in Applied Soil Ecology, 22(2) February 2003, p. 193: "The authors' regret that an error occurred in the first sentence of Section 2.4. This sentence is reproduced correctly below. DGGE bands that appeared visually to be either lost or gained due to the application of chlorothalonil, were excised, eluted in 100 μl water, re-amplified as described above, and electrophoresed with DGGE."
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Sigler, W. V., and R. F. Turco. 2002. The impact of chlorothalonil application on soil bacterial and fungal populations as assessed by denaturing gradient gel electrophoresis. Applied Soil Ecology. 21(2):p. 107-118. |
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| DOI: 10.1016/S0929-1393(02)00088-4 |
| Web URL(s):
http://www.sciencedirect.com/science/article/pii/S0929139302000884
Last checked: 05/29/2014
Access conditions: Item is within a limited-access website |
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MSU catalog number: QH541.5.S6 Online |
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