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| DOI: | 10.1093/aob/mcr083 |
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| Web URL(s): | https://academic.oup.com/aob/article/107/8/1313/173531
Last checked: 07/09/2018
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Harper, John;
Armstead, Ian;
Thomas, Ann;
James, Caron;
Gasior, Dagmara;
Bisaga, Maciej;
Roberts, Luned;
King, Ian;
King, Julie |
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| Author Affiliation: | Harper, Armstead, Thomas, James, Gasior, Bisaga and Roberts: Institute of Biological, Environmental and Rural Sciences, Aberystwyth Universitmy,Gogerddan, Aberyswyth, Cerediqion, UK; Ian King and Julie King: Division of Plant and Crop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington, UK |
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| Title: | Alien introgression in the grasses Lolium perenne (perennial ryegrass) and Festuca pratensis (meadow fescue): The development of seven monosomic substitution lines and their molecular and cytological characterization |
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| Section: | Original articles
Other records with the "Original articles" Section
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| Source: | Annals of Botany. Vol. 107, No. 8, June 2011, p. 1313-1321. |
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| Publishing Information: | London, Oxford University Press |
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| # of Pages: | 9 |
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| Keywords: | TIC Keywords: Cereals; Chromosome fragments; Chromosome morphology; Festuca pratensis; Gene isolation; Genetic characterization; Interspecific hybridization; Introgression; Lolium perenne; Molecular markers
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| Abstract/Contents: | "Background and Aims To address the issues associated with food security, environmental change and bioenergy in the context of crop plants, the production, identification and evaluation of novel plant phenotypes is fundamental. One of the major routes to this end will be wide hybridization and introgression breeding. The transfer of chromosomes and chromosome segments between related species (chromosome engineering or alien introgression) also provides an important resource for determining the genetic control of target traits. However, the realization of the full potential of chromosome engineering has previously been hampered by the inability to identify and characterize interspecific introgressions accurately. Methods Seven monosomic substitution lines have been generated comprising Festuca pratensis as the donor species and Lolium perenne as the recipient. Each of the seven lines has a different L. perenne chromosome replaced by the homoeologous F. pratensis chromosome (13 L.perenne + 1 F. pratensis chromosome). Molecular markers and genomic in situ hybridization (GISH) were used to assign the F. pratensis chromosomes introgressed in each of the monosomic substitutions to a specific linkage group. Cytological observations were also carried out on metaphase I of meiosis in each of the substitution lines. Results A significant level of synteny was found at the macro-level between L. perenne and F. pratensis. The observations at metaphase I revealed the presence of a low level of interspecific chromosomal translocations between these species. Discussion The isolation of the seven monosomic substitution lines provides a resource for dissecting the genetic control of important traits and for gene isolation. Parallels between the L. perenne/F. pratensis system and the Pooideae cereals such as wheat, barely, rye, oats and the model grass Brachypodium distachyon present opportunities for a comparison across the species in terms of genotype and phenotype." |
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| Language: | English |
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| References: | 56 |
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| Note: | Pictures, b/w
Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Harper, J., I. Armstead, A. Thomas, C. James, D. Gasior, M. Bisaga, et al. 2011. Alien introgression in the grasses Lolium perenne (perennial ryegrass) and Festuca pratensis (meadow fescue): The development of seven monosomic substitution lines and their molecular and cytological characterization. Ann. Bot. 107(8):p. 1313-1321. |
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| DOI: 10.1093/aob/mcr083 |
| Web URL(s):
https://academic.oup.com/aob/article/107/8/1313/173531
Last checked: 07/09/2018
Access conditions: Item is within a limited-access website |
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MSU catalog number: b2209341a |
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