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| DOI: | 10.1007/s11032-004-7243-z |
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| Web URL(s): | https://link.springer.com/content/pdf/10.1007%2Fs11032-004-7243-z
Last checked: 10/05/2017
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Chen, Xi;
Yang, Wenqian;
Sivamani, Elumalai;
Bruneau, Art H.;
Wang, Binghe;
Qu, Rongda |
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| Author Affiliation: | Chen, Sivamani, Brunea, and Qu: Department of Crop Science; Yang and Wang: Department of Chemistry, North Carolina State University, Raleigh, NC; Yang: IRIX Pharmaceuticals, Inc., Florence, SC; Wang: Department of Chemistry, Georgia State University, Atlanta, GA |
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| Title: | Selective elimination of perennial ryegrass by activation of a pro-herbicide through engineering E. coli argE gene |
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| Source: | Molecular Breeding. Vol. 15, No. 4, May 2005, p. 339-347. |
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| Publishing Information: | The Netherlands: Kluwer Academic Publishers |
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| # of Pages: | 9 |
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| Related Web URL: | http://www.springerlink.com/content/x17jj475863pj409/
Last checked: 03/28/2012
Notes: Abstract only |
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| Keywords: | TIC Keywords: Control methods; Genetic control; Glufosinate; Lolium perenne; Overseeding; Selective herbicides; Transgenes
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| Abstract/Contents: | "Perenniel ryegrass is widely used for overseeding dormant bermudagrass on golf courses and sports fields in Southeastern United States to provide green color and improved playability. Late spring and summer persistence of perennial ryegrass may decrease the quality of the bermudagrass turf and reduce its winter hardiness. To help solve this problem, we developed a strategy to activate a pro-herbicide within the transgenic perennial ryegrass plants and to cause self elimination of the plants. An E. coli argE gene was introduced into perennial ryegrass by the biolistic method, which resulted in four independently transformed green plants. The mRNA of argE gene was detected in three of the plants by RT-PCR. Perennial ryegrass plants expressing the argE transgene were selectively controlled upon application of a pro-herbicide, N-acetyl-l-phosphinothricin (or N-acetyl-PPT), since the N-acetylornithinase encoded by argE gene is able to convert N-acetyl-PPT to the herbicide phosphinothricin (PPT). The non-transgenic bermudagrass plants were unaffected by the treatment. This approach provides a means to selectively remove a group of transgenic plants without affecting other plants growing with them." |
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| Language: | English |
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| References: | 25 |
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| Note: | Pictures, color |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Chen, X., W. Yang, E. Sivamani, A. H. Bruneau, B. Wang, and R. Qu. 2005. Selective elimination of perennial ryegrass by activation of a pro-herbicide through engineering E. coli argE gene. Molecular Breeding. 15(4):p. 339-347. |
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| DOI: 10.1007/s11032-004-7243-z |
| Web URL(s):
https://link.springer.com/content/pdf/10.1007%2Fs11032-004-7243-z
Last checked: 10/05/2017
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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