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| Web URL(s): | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5247334/?report=classic
Last checked: 06/27/2017
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5247334/pdf/297.pdf#page=9
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http://journals.fcla.edu/jon/article/view/90512/86808
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| Publication Type:
| Refereed |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Blauel, T.;
Wallace, T.;
VanDyk, D.;
Celetti, M.;
McDonald, M. R.;
Jordan, K. S. |
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| Author Affiliation: | Plant Agriculture Dept., University of Guelph, Guelph, ON |
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| Title: | Comparison of extraction methods to accurately estimate plant-parasitic nematodes from a variety of soils |
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| Section: | Abstracts
Other records with the "Abstracts" Section
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| Source: | Journal of Nematology. Vol. 48, No. 4, December 2016, p. 305-306. |
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| Publishing Information: | Lawrence, Kansas: Society of Nematologists |
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| # of Pages: | 2 |
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| Keywords: | TIC Keywords: Comparisons; Extraction; Methods; Nematode surveys; Pest profile; Plant parasitic nematodes; Soil analysis
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| Abstract/Contents: | "Various management decisions regarding plant-parasitic nematodes are often made based on screening of soils for the presence of nematodes either prior to planting or as a result of unexplained symptoms on the plants. Published threshold levels are also used to determine whether or not to apply nematicides or even to fumigate soils to prevent nematode damage during a growing season. However, inconsistencies between laboratories has made it difficult for growers to have confidence in soil test results. Additionally, the traditional method of soil nematode extraction, the Baermann pan (BP) method, only extracts live, motile nematodes. It is possible that slow-moving genera are being excluded from counts or are being underestimated due to the nature of the extraction method. In order to extract the most nematodes, the BP method requires days before counts can be made. The sugar centrifugation (SC) method for nematode extraction from soils extracts both living and non-living nematodes but can also be used to estimate populations of slow-moving or non-motile genera in the soil. Another advantage is that the extraction and counts can all be done the same day that samples are collected, allowing growers to obtain a faster response. Although most diagnostic laboratories in Canada use the BP method for soil nematode extraction, it is believed that for certain genera the SC method more accurately estimates population levels. A series of studies were undertaken to compare nematode extraction methods from soil to ensure that current recommendations are based on the most accurate predictions of nematode populations in the soil. Samples from turfgrass (representing turfgrass nematode genera and sandy soils), carrot (representing carrot genera and high organic matter (muck) soils) and tomato (representing tomato nematode genera and fine-textured soils) were collected and subjected to both the BP and SC soil nematode extraction methods. From turfgrass soils, 3 golf greens sites were selected at 4 different golf courses for comparison. From the carrot soils samples from five different fields were collected and from the tomato fields, samples from ten different fields were collected for comparison. All samples were homogenized prior to removing 50cc aliquots of soil for extraction. In the turfgrass samples, the SC method extracted significantly more nematodes within the Heterodera, Meloidogyne, Criconemoides, Helicotylenchus and Tylenchorhynchus genera but there was no significant difference between the two methods for free-living nematodes. In the muck soil samples, the SC method extracted significantly more nematodes within the Heterodera genus but significantly fewer Paratylenchus nematodes. Additional analysis from carrot and tomato soils is currently underway." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Blauel, T., T. Wallace, D. VanDyk, M. Celetti, M. R. McDonald, and K. S. Jordan. 2016. Comparison of extraction methods to accurately estimate plant-parasitic nematodes from a variety of soils. J. Nematol. 48(4):p. 305-306. |
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| Web URL(s):
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5247334/?report=classic
Last checked: 06/27/2017
Notes: Item is within a single large file
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5247334/pdf/297.pdf#page=9
Last checked: 06/27/2017
Requires: PDF reader
Notes: Item is within a single large file
http://journals.fcla.edu/jon/article/view/90512/86808
Last checked: 08/17/2018
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: b2224870a |
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