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| Web URL(s): | https://archive.lib.msu.edu/tic/its/articles/2005jou485.pdf
Last checked: 09/29/2008
Requires: PDF Reader |
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| Access Restriction: | Certain MSU-hosted archive URLs may be restricted to legacy database members. |
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| Publication Type:
| Refereed |
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| Author(s): | Altpeter, F.;
James, V. A. |
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| Author Affiliation: | University of Florida - IFSA, Agronomy Department, PMCB, Laboratory of Molecular Plant Physiology, Gainesville, Florida |
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| Title: | Genetic transformation of turf-type bahiagrass (Paspalum notatum flugge) by biolistic gene transfer |
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| Section: | Genetics, breeding & variety evaluation
Other records with the "Genetics, breeding & variety evaluation" Section
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| Meeting Info.: | Llandudno, Wales, UK: July 10-15 2005 |
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| Source: | International Turfgrass Society Research Journal. Vol. 10, No. Part 1, 2005, p. 485-489. |
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| Publishing Information: | Aberystywth, Ceredigion, UK: International Turfgrass Society |
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| # of Pages: | 5 |
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| Keywords: | TIC Keywords: Genetic transformation; Paspalum notatum; Genetic engineering; Turfgrass quality; Cultivar improvement; Tissue culture; Transgenic turfgrasses; Calli; Gene expression; Regeneration; Polymerase chain reaction
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| Cultivar Names: | Argentine |
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| Abstract/Contents: | "Bahiagrass (Paspalum notatum Flugge) is extensively used in the southern USA for utility turf along highways and for residential lawns. The popularity of bahiagrass is attributed to its excellent persistence supported by drought tolerance, heat tolerance, insect and disease resistance, and nematode suppression. Genetic engineering of turf-type bahiagrass is desirable to improve turf quality. The cultivar, 'Argentine', was selected for genetic transformation because it is a commercially important turf-type bahiagrass cultivar and its tetraploid, apomictic nature should result in uniformity of the transgenic progeny and transgene containment due to sexual incompatibility with diploid wildtype bahiagrass. We recently developed an efficient tissue culture system for the regeneration of 'Argentine' turf-type bahiagrass from mature seed-derived callus. Biolistic gene transfer of a constitutive nptII expression cassette into seed-drived callus cultures was followed by selection with 50 mg 1-1 paromomycin sulfate during callus subculture and regeneration of plantlets. Transgenic plants were transferred to soil in approximately 17 to 20 weeks from the initiation of mature seed cultures. The regenerated plants were evaluated with PCR of genomic DNA, using primers annealing to the transgene expression cassette and ELISA of leaf protein extracts using a NPTII antibody." |
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| Language: | English |
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| References: | 17 |
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| Note: | Pictures, b/w
Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Altpeter, F., and V. A. James. 2005. Genetic transformation of turf-type bahiagrass (Paspalum notatum flugge) by biolistic gene transfer. Int. Turfgrass Soc. Res. J. 10(Part 1):p. 485-489. |
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| Web URL(s):
https://archive.lib.msu.edu/tic/its/articles/2005jou485.pdf
Last checked: 09/29/2008
Requires: PDF Reader |
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MSU catalog number: SB 433 .I52 v. 10 |
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