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| DOI: | 10.1111/j.1365-3180.2005.00467.x |
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| Web URL(s): | http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3180.2005.00467.x/full
Last checked: 01/31/2014
Access conditions: Item is within a limited access website
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3180.2005.00467.x/pdf
Last checked: 01/31/2014
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| Publication Type:
| Refereed |
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| Author(s): | Délye, C.;
Michel, S. |
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| Author Affiliation: | Institut National de la Recherche Agronomique, UMR Biologie et Gestion des Adventices, Dijon Cédex, France |
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| Title: | 'Universal' primers for PCR-sequencing of grass chloroplastic acetyl-CoA carboxylase domains involved in resistance to herbicides |
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| Source: | Weed Research. Vol. 45, No. 5, October 2005, p. 323-330. |
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| Publishing Information: | Oxford: Blackwell Scientific Publications |
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| # of Pages: | 8 |
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| Related Web URL: | http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3180.2005.00467.x/abstract
Last checked: 01/31/2014
Notes: Abstract only |
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| Keywords: | TIC Keywords: Herbicides; Polymerase chain reaction; Acetyl-CoA carboxylase inhibitors; Poaceae; Acetyl-coenzyme A; Resistance; Genetics; Herbicide resistance; Wetting agents
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| Abstract/Contents: | "Primers were designed to amplify two regions involved in sensitivity to herbicides inhibiting the plastidic acetyl-CoA carboxylase (ACCase) from grasses (Poaceae). The first primer pair amplified a 551-bp amplicon containing a variable Ile/Leu codon at position 1781 in Alopecurus myosuroides sequence. The second primer pair amplified a 406-bp amplicon containing four variable codons (Trp/Cys, Ile/Asn, Asp/Gly, Gly/Ala) at positions 2027, 2041, 2078 and 2096, respectively, in A. myosuroides sequence. Both primer pairs amplified the targeted fragments from genes encoding plastidic ACCases, but not from the very similar genes encoding cytosolic ACCases. Clear DNA sequences were obtained from fresh or dried plant material from the field, and from 29 various grass species. Sequences revealed that the gene encoding plastidic ACCase in Poa annua and Festuca rubra contained a Leu1781 codon, in agreement with both species being inherently tolerant to herbicides inhibiting ACCase. Sequencing confirmed the hybird origin of P. annua. Compared with ACCase enzyme assay, polymerase chain reaction is faster, can be performed from a single plant and suppresses the need for radioactive experiments. It can be completed with basic molecular biology laboratory equipment. It is the tool of choice for diagnosing resistance caused by alteration(s) of the plastidic ACCase." |
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| Language: | English |
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| References: | 34 |
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| See Also: | Other items relating to: Wetting Agents |
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| Note: | Pictures, b/w
Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Délye, C., and S. Michel. 2005. 'Universal' primers for PCR-sequencing of grass chloroplastic acetyl-CoA carboxylase domains involved in resistance to herbicides. Weed Res. 45(5):p. 323-330. |
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| DOI: 10.1111/j.1365-3180.2005.00467.x |
| Web URL(s):
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3180.2005.00467.x/full
Last checked: 01/31/2014
Access conditions: Item is within a limited access website
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3180.2005.00467.x/pdf
Last checked: 01/31/2014
Requires: PDF Reader
Access conditions: Item is within a limited access website |
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MSU catalog number: SB 599 .W4 |
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