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| Publication Type:
| Refereed |
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| Author(s): | Henry, Robert, J.;
Stone, Bruce, A. |
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| Author Affiliation: | Department of Biochemistry, La Trobe University, Bundoora, Victoria, Australia |
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| Title: | Factors influencing ß-glucan synthesis by particulate enzymes from suspension-cultured Lolium multiflorum endosperm cells |
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| Source: | Plant Physiology. Vol. 69, 1982, p. 632-636. |
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| Publishing Information: | Rockville, MD: American Society of Plant Physiologists |
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| # of Pages: | 5 |
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| Keywords: | TIC Keywords: Temperatures; pH; Ions; Inhibitors; Activators; Lolium multiflorum; Enzymes; Glucose
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| Abstract/Contents: | "Particulate enzymes from suspension-cultured ryegrass (Lolium multiflorum Lam.) endosperm cells incorporated glucosyl residues from UDP-glucose and GDP-glucose into ß-glucans. Three types of ß-glucans were produced from UDP-glucose: 1,3-ß-glucan; 1,4-ß-glucan; and mixed-linkage 1,3;1,4-ß-glucan. As in other systems, relatively more 1,4-ß-glucan was produced from a low (10 micromolar) UDP-glucose concentration, and relatively more 1,3-ß-glucan was produced from a high (1 millimolar) UDP-glucose concentration. However, in ryegrass 1,3;1,4-ß-glucan represented a major proportion of the products at both low and high UDP-glucose concentrations. The arrangement of linkages in the 1,3;1,4-ß-glucan was different at the two concentrations; at the low UDP-glucose concentration, more sequences of three consecutive 1,4-linkages were produced. The effects of pH, temperature, and metal ion concentrations on incorporation were dependent on the UDP-glucose concentration. At the low UDP-glucose concentration, incorporation into all three types of ß-glucan increased with increasing pH. At the high UDP-glucose concentration, 1,3-ß-glucan was the major product at pH 7 and below; 1,4-ß-glucan synthesis was optimal at pH 8; and synthesis of 1,3;1,4-ß-glucan was greatest above pH 8. With 10 micromolar GDP-glucose as substrate, 1,4-ß-glucan, but no 1,3;1,4-ß-glucan, was produced. Incorporation from either UDP-glucose of GDP-glucose was not influenced by the presence or the other." |
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| Language: | English |
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| References: | 29 |
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| Note: | Graphs
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Henry, R. J., and B. A. Stone. 1982. Factors influencing ß-glucan synthesis by particulate enzymes from suspension-cultured Lolium multiflorum endosperm cells. Plant Physiol. 69:p. 632-636. |
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