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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2006.96.6.S1#page=36
Last checked: 10/20/2015
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Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Fouly, H. M.;
Park, J.;
Henning, S.;
Wilkinson, H. T. |
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| Author Affiliation: | University of Illinois, Urbana, Illinois |
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| Title: | Use of GUS transformants of Gaeumannomyces graminis var. graminis to study the role of melanin during the pathogenesis of grass roots |
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| Section: | APS abstracts submitted for presentation at the 2006 APS Annual Meeting
Other records with the "APS abstracts submitted for presentation at the 2006 APS Annual Meeting" Section
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| Source: | Phytopathology. Vol. 96, No. 6, June Supplement 2006, p. S36-S37. |
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| Publishing Information: | St. Paul, MN: American Phytopathological Society |
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| # of Pages: | 2 |
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| Keywords: | TIC Keywords: Ground water ubiquity score; Gaeumannomyces graminis var. graminis; Pythium diseases; Hyphae; Disease profile
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| Abstract/Contents: | "Gaeumannomyces graminis var. graminis (Ggg) causes root rot of turfgrass and sheath blight of rice. During pathogenesis, the fungus produces ectotrophic, melanized hyphae (runner hyphae) which differentiate at their tips to produce a melanized, lobed hyphopodim (appressoria). Hyphopodia produce penetration structures, and upon ingress invade epidermal and cortical tissues with narrow, hyaline hyphae. When the infecting hyphae cross the endodermis and invade the stele, they re-initiate melanin production and become thicker. It appears that the invasion of the stele is coincidental with external stresses of heat and/or drought. A wild-type isolate of Ggg was transformed with the GUS (beta-glucuronidase) reporter and hygromycin (hph) resistance genes. Of five stable hygromycin-resistant transformants, only one transformant was resistant to hygromycin and unable to produce melanin. GUS activity was quantified for the transfomants: whereas no activity was recorded for the wild-type isolate. A southern hybridization analysis verified integration of the hph gene and the GUS gene into the transformant's genomes. Histopathological studies suggest that non-melanized hyphae are not as efficient as melanized hyphae in gaining ingress or colonization." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Fouly, H. M., J. Park, S. Henning, and H. T. Wilkinson. 2006. Use of GUS transformants of Gaeumannomyces graminis var. graminis to study the role of melanin during the pathogenesis of grass roots. Phytopathology. 96(6):p. S36-S37. |
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| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2006.96.6.S1#page=36
Last checked: 10/20/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: SB 599 .P48 |
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