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| Web URL(s): | http://turf.rutgers.edu/research/abstracts/symposium2007.pdf#page=53
Last checked: 11/28/2007
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Rotter, David;
Warnke, Scott;
Belanger, Faith C. |
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| Author Affiliation: | Rotter, and Belanger: Department of Plant Biology and Pathology, Rutgers University; Warnke: United States Department of Agriculture-Agricultural Research Service, Beltsville, Maryland |
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| Title: | Dideoxy polymorphism scanning, an efficient gene-based method for marker development |
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| Section: | Poster presentations
Other records with the "Poster presentations" Section
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| Meeting Info.: | Cook College, Rutgers, NJ: January 11-12, 2007 |
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| Source: | Proceedings of the Sixteenth Annual Rutgers Turfgrass Symposium. Vol. 16, 2007, p. 52. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Gene mapping; Genetic markers; Agrostis tenuis; Dideoxy polymorphism scanning; Single nucleotide polymorphism; Amplification fragment length polymorphisms
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| Abstract/Contents: | "Genetic linkage mapping and QTL analysis of important phenotypic traits is currently an active area of research in agronomically important plants and animals. Marker assisted breeding, based on linkage maps and QTLs, is likely to be one of the most important and broadly useful applications of biotechnology in agriculture. The ultimate goal of genetic linkage mapping is to identify the genes controlling important phenotypic traits. However, effective breeding strategies can be developed prior to gene identification based on closely linked markers. Current genetic linkage mapping uses molecular markers almost exclusively and is based on DNA sequence polymorphisms between parents whose segregation is followed in their progeny. Numerous types of molecular markers have been used to develop linkage maps, such as restriction fragment length polymorphisms (RFLPs), randomly amplified polymorphic DNAs (RAPDs), amplified fragment length polymorphisms (AFLPs), and simple sequence repeats (SSRs). To facilitate the association of phenotype with genes, and for comparative genomics, gene-based maps are highly desirable. With the rapidly increasing availability of expressed sequence tag (EST) sequences have been reported. Single nucleotide polymorphisms (SNPs) and small indels are the most widespread types of polymorphisms in both plant and animal genomes, and SNPs are becoming the marker type of choice. Although SNPs and indels are relatively common, their use in the development of gene-based markers for mapping can be difficult. To improve the efficiency of marker generation, we have developed a simple and cost effective method of polymorphism detection. We refer to this method as dideoxy polymorphism scanning (ddPS0 (Rotter et al, 2006). We are currently using the ddPS method in developing a genetic linkage map of colonial bentgrass. Our current map covers 800 cM and consists of 15 linkage groups. Additional markers should resolve the linkage groups into the expected 14, 7 for the A1 genome and 7 for the A2 genome. We now have a total of 104 linked markers (43 gene based and 61 AFLP) and 37 unlinked markers (18 geen based and 19 AFLP)." |
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| Language: | English |
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| References: | 1 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Rotter, D., S. Warnke, and F. C. Belanger. 2007. Dideoxy polymorphism scanning, an efficient gene-based method for marker development. Proc. Annu. Rutgers Turfgrass Symp. 16:p. 52. |
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| Web URL(s):
http://turf.rutgers.edu/research/abstracts/symposium2007.pdf#page=53
Last checked: 11/28/2007
Requires: PDF Reader |
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MSU catalog number: SB 433 .R88 |
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