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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Oh, Chan-Seok;
Hillman, Bradley I. |
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| Author Affiliation: | Department of Plant Pathology, Rutgers University, New Brunswick, New Jersey |
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| Title: | Screening and characterization of extrachromosomal genetic elements in turfgrass endophytes |
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| Section: | Poster presentations
Other records with the "Poster presentations" Section
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| Meeting Info.: | Cook College, Rutgers, NJ: January 15-16, 1993 |
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| Source: | Proceedings of the Second Annual Rutgers TurfgrassSymposium. Vol. 2, 1993, p. 23. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Endophytes; Fungi; Acremonium; Atkinsonella hypoxylon; Ribonucleic acid; Characteristics; Amino acids; Cycloheximide
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| Abstract/Contents: | "The objectives of this project are to examine turfgrass endophytes for extrachromosomal genetic elements such as double-stranded (ds) RNAs or plasmids that may have an effect on the biology of the fungus or that may be useful for transformation. Biological effects include attributes such as the ability to colonize, produce alkaloids, or cause disease in the host. To date, no dsRNA elements have been identified in the 36 isolates of various Acremonium species that have been examined, but dsRNA elements were identified in Atkinsonella hypoxylon, a related epiphyte that causes choke disease on Danthonia spicata (poverty grass) but has an overall positive effect on vegetative growth of the grass, propably due to production of alkaloids. Subsequently, five isolates of A. hypoxylon from New Brunswick and one from Indiana have been examined for extrachromosomal elements, and all have been found to contain them. The dsRNAs vary in size (0.3-3.0 kbp), numbers of segments (1-9), and accumulation (0.5-10 μg/g tissue). A library of complimentary DNA clones representing the three resident dsRNAs of one of the isolates has been synthesized, mapped, and partially sequenced, including the almost complete sequencing of one of the three segments. On that segment, a single large open reading frame has been identified. Comparisons of the amino acid sequence deduced from this large open reading frame has revealed no similarities with other proteins in the date base, including studies directed toward higher scrutiny of other fungal dsRNA sequences. Efforts to cure this strain of its resident extrachromosomal dsRNA elements by single sporing have thus far been unsuccessful, as the elements have passed through 100% of single conidia examined to date. Cycloheximide treatment, which cures fungi of some dsRNA elements, also failed to cure A. hypoxylon." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Oh, C., and B. I. Hillman. 1993. Screening and characterization of extrachromosomal genetic elements in turfgrass endophytes. Proc. Annu. Rutgers Turfgrass Symp. 2:p. 23. |
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