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Web URL(s): | http://img.kisti.re.kr/soc_img/society//tsk/JDHHBF/1998/v12n4/JDHHBF_1998_v12n4_195.pdf Last checked: 01/28/2013 Requires: PDF Reader |
Publication Type:
| Refereed |
Author(s): | Chai, Ming Liang;
Lee, Jong Min;
Kim, Doo Hwan |
Author Affiliation: | Department of Horticultural Science, Konkuk University, Seoul, Korea |
Title: | High efficiency of plant regeneration from seed-derived callus of zoysiagrass cv. zenith |
Source: | Korean Journal of Turfgrass Science. Vol. 12, No. 4, 1998, p. 195-202[676-683]. |
Publishing Information: | Korea: Turfgrass Society of Korea |
# of Pages: | 8 |
Keywords: | TIC Keywords: Regeneration; Calli; Zoysia Japonica; Breeding improvement; Seeds
|
Cultivar Names: | Zenith |
Abstract/Contents: | "The development of a protocol for efficiency of embryogenic callus separation, maintenance and plant regeneration fron seeds of zoysiagrass cv. Zenith was studied. Embryogenic callus ratio is absolutely determined by genotype, but by adding high concentration of copper into medium, changing light condition and maintaining callus on initial induction medium for 8~10 weeks, embryogenic callus can be easily distinguished and its growth can be promoted. There were significant differences among selected callus lines (each from one seed) according to their growth rates and regeneration percentages. Callus pre-treatment with activated charcoal inhibited callus growth, increased the level of precocious germination during culture and promoted shoot cluster formation after transfer to regeneration medium. For long-term callus maintenance, N6AA medium was better than MS medium, because the former inhibited non-embryogenic callus formation and kept vigor of embryogenic callus. The best callus lines Z-(5) has been successfully used for transformation and somaclonal variation selection in our laboratory." |
Language: | English |
References: | 11 |
Note: | Abstract appears in English Pictures, b/w Tables Graphs |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Chai, M. L., J. M. Lee, and D. H. Kim. 1998. High efficiency of plant regeneration from seed-derived callus of zoysiagrass cv. zenith. (In English, with English abstract.) Korean Journal of Turfgrass Science. 12(4):p. 195-202[676-683]. |
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| Web URL(s): http://img.kisti.re.kr/soc_img/society//tsk/JDHHBF/1998/v12n4/JDHHBF_1998_v12n4_195.pdf Last checked: 01/28/2013 Requires: PDF Reader |
| MSU catalog number: b5472572a |
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