Full TGIF Record # 134499
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Web URL(s):http://turf.rutgers.edu/research/abstracts/symposium2008.pdf
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Publication Type:
i
Report
Content Type:Abstract or Summary only
Author(s):Chi, Emily; Clarke, Bruce B.; Hillamn, Bradley I.; Crouch, Jo Anne
Author Affiliation:Department of Plant Biology and Pathology, Rutgers University, New Brunswick, New Jersey
Title:Virus populations of Sclerotinia homoeocarpa, causal agent of dollar spot disease on turfgrass
Section:Poster presentations
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Meeting Info.:New Brunswick, NJ: January 10-11, 2008
Source:Proceedings of the Seventeenth Annual Rutgers TurfgrassSymposium. 2008, p. 39.
Publishing Information:New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey
# of Pages:1
Keywords:TIC Keywords: Viruses; Sclerotinia homoeocarpa; Dollar spot; Reproduction; Phenotypes
Abstract/Contents:"The taxonomically unresolved pathogen currently known as Sclerotinia homoeocarpa is the causal agent of dollar spot, the most common disease of cool-season turfgrasses in North America. The pathogen's primary mode of replication is clonal, with disease arising through overwintering stroma or systemically infected host tissue; further dissemination of human-mediated. Fertile apothecia, the sexual structure of the fungus that contains asci and ascospores, have not been reported from North American isolates. Interspecific and intraspecific genetic exchange can be mediated by hyphal anastomosis, of fusion. In previous studies of S. homoeocarpa, a 2.6kbo dsRNA was identified from the SH12b isolate conspecific with Ophiostoma mitovirus 3a-OnuLd (OMV3a-OnuLd) that displayed an aberrant colony and morphology and hypovirulent (less virulent than wildtype) phenotype. OMV3a-Sh12B was successfully utilized in pilot biocontrol studies of dollar spot on creeping bentgrass in both field and controlled experimental conditions. Although this may prove to be an important avenue to pursue in the future, biocontrol is not an explicit goal of our research. Our interest for this project is to determine the relationship between different virus' fungal phenotype and distribution as a means of shedding light on the effect viruses have on the evolution of S. homoeocarpa. Virus populations are to be represented by isolatable dsRNAs. The current sample pool consists of forty cultures of S. homoeocarpa isolated from six different grasses grown in five states. dsRNA extracts from four randomly selected isolated produced unique PAGE banding patterns. Confirmed dsRNAs will be reverse-transcribed and cloned by PCR, and viral cDNA libraries will be created and analyzed in silico. Isolated that contain dsRNA and display an aberrant phenotype will be stored for future biological studies.
Language:English
References:0
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Chi, E., B. B. Clarke, B. I. Hillman, and J. A. Crouch. 2008. Virus populations of Sclerotinia homoeocarpa, causal agent of dollar spot disease on turfgrass. Proc. Annu. Rutgers Turfgrass Symp. p. 39.
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http://turf.rutgers.edu/research/abstracts/symposium2008.pdf
    Last checked: 11/05/2015
    Requires: PDF Reader
    Notes: Item is within a single large file
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MSU catalog number: SB 433 .R88
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