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DOI: | 10.2135/cropsci2007.10.0597 |
Web URL(s): | https://dl.sciencesocieties.org/publications/cs/pdfs/48/5/1881 Last checked: 11/16/2016 Requires: PDF Reader Access conditions: Item is within a limited-access website https://dl.sciencesocieties.org/publications/cs/articles/48/5/1881 Last checked: 11/16/2016 Access conditions: Item is within a limited-access website |
Publication Type:
| Refereed |
Author(s): | Martin, Ruth C.;
Hollenbeck, Vicky G.;
Dombrowski, James E. |
Author Affiliation: | United States Department of Agriculture - Agricultural Research Service, National Forage Seed Production Research Center, Corvallis, Oregon |
Title: | Evaluation of reference genes for quantitative RT-PCR in Lolium perenne |
Section: | Genomics molecular genetics & biotechnology Other records with the "Genomics molecular genetics & biotechnology" Section
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Source: | Crop Science. Vol. 48, No. 5, September/October 2008, p. 1881-1887. |
Publishing Information: | Madison, WI: Crop Science Society of America |
# of Pages: | 7 |
Related Web URL: | https://dl.sciencesocieties.org/publications/cs/abstracts/48/5/1881 Last checked: 11/16/2016 Notes: Abstract only |
Keywords: | TIC Keywords: Genetics; Evaluations; Lolium perenne; Genes; Reverse transcription-ploymerase chain reaction; Gene expression; Growth factors
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Abstract/Contents: | "Quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) provides an important tool for analyzing gene expression if proper internal standards are used. The aim of this study was to identify and evaluate reference genes for use in real-time quantitative RT-PCR in perennial ryegrass (Lolium perenne L.) during plant development. Partial sequences of nine L. perenne housekeeping genes were obtained by RT-PCR using degenerate primers designed from the corresponding genes in closely related species. Primers for quantitative RT-PCR were designed based on partial sequences. The housekeeping genes were evaluated for thier expression stabililty in different tissues at various stages of development. The analysis found that eEF-1α and eIF-4α were the most stable and ß-TUB was the least stable of the genes tested when all tissues were analyzed together. Analysis by geNorm indicated that the four most stably expressed housekeeping genes (eEF-1α, eIF-4α, 25S rRNA, and GAPDH) should be utilized when normalizing gene expression during plant developmental studies. For root crown tissues at different stages of development, eIF-4α and 25S rRNA were the most stably expressed of the housekeeping genes tested. In leaf tissues, eEF-1α and UBQ5 were the most stably expressed of the housekeeping genes tested. We found that using two housekeeping genes as reference genes is sufficient during RT-PCR gene expression studies when analyzing either root crown or leaf tissues during different stages of development." |
Language: | English |
References: | 24 |
Note: | Tables Graphs |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Martin, R. C., V. G. Hollenbeck, and J. E. Dombrowski. 2008. Evaluation of reference genes for quantitative RT-PCR in Lolium perenne. Crop Sci. 48(5):p. 1881-1887. |
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| DOI: 10.2135/cropsci2007.10.0597 |
| Web URL(s): https://dl.sciencesocieties.org/publications/cs/pdfs/48/5/1881 Last checked: 11/16/2016 Requires: PDF Reader Access conditions: Item is within a limited-access website https://dl.sciencesocieties.org/publications/cs/articles/48/5/1881 Last checked: 11/16/2016 Access conditions: Item is within a limited-access website |
| MSU catalog number: b2211522a |
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