Full TGIF Record # 140000
Item 1 of 1
DOI:10.2135/cropsci2007.10.0597
Web URL(s):https://dl.sciencesocieties.org/publications/cs/pdfs/48/5/1881
    Last checked: 11/16/2016
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https://dl.sciencesocieties.org/publications/cs/articles/48/5/1881
    Last checked: 11/16/2016
    Access conditions: Item is within a limited-access website
Publication Type:
i
Refereed
Author(s):Martin, Ruth C.; Hollenbeck, Vicky G.; Dombrowski, James E.
Author Affiliation:United States Department of Agriculture - Agricultural Research Service, National Forage Seed Production Research Center, Corvallis, Oregon
Title:Evaluation of reference genes for quantitative RT-PCR in Lolium perenne
Section:Genomics molecular genetics & biotechnology
Other records with the "Genomics molecular genetics & biotechnology" Section
Source:Crop Science. Vol. 48, No. 5, September/October 2008, p. 1881-1887.
Publishing Information:Madison, WI: Crop Science Society of America
# of Pages:7
Related Web URL:https://dl.sciencesocieties.org/publications/cs/abstracts/48/5/1881
    Last checked: 11/16/2016
    Notes: Abstract only
Keywords:TIC Keywords: Genetics; Evaluations; Lolium perenne; Genes; Reverse transcription-ploymerase chain reaction; Gene expression; Growth factors
Abstract/Contents:"Quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) provides an important tool for analyzing gene expression if proper internal standards are used. The aim of this study was to identify and evaluate reference genes for use in real-time quantitative RT-PCR in perennial ryegrass (Lolium perenne L.) during plant development. Partial sequences of nine L. perenne housekeeping genes were obtained by RT-PCR using degenerate primers designed from the corresponding genes in closely related species. Primers for quantitative RT-PCR were designed based on partial sequences. The housekeeping genes were evaluated for thier expression stabililty in different tissues at various stages of development. The analysis found that eEF-1α and eIF-4α were the most stable and ß-TUB was the least stable of the genes tested when all tissues were analyzed together. Analysis by geNorm indicated that the four most stably expressed housekeeping genes (eEF-1α, eIF-4α, 25S rRNA, and GAPDH) should be utilized when normalizing gene expression during plant developmental studies. For root crown tissues at different stages of development, eIF-4α and 25S rRNA were the most stably expressed of the housekeeping genes tested. In leaf tissues, eEF-1α and UBQ5 were the most stably expressed of the housekeeping genes tested. We found that using two housekeeping genes as reference genes is sufficient during RT-PCR gene expression studies when analyzing either root crown or leaf tissues during different stages of development."
Language:English
References:24
Note:Tables
Graphs
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Martin, R. C., V. G. Hollenbeck, and J. E. Dombrowski. 2008. Evaluation of reference genes for quantitative RT-PCR in Lolium perenne. Crop Sci. 48(5):p. 1881-1887.
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DOI: 10.2135/cropsci2007.10.0597
Web URL(s):
https://dl.sciencesocieties.org/publications/cs/pdfs/48/5/1881
    Last checked: 11/16/2016
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
https://dl.sciencesocieties.org/publications/cs/articles/48/5/1881
    Last checked: 11/16/2016
    Access conditions: Item is within a limited-access website
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