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DOI: | 10.1094/PDIS-93-9-0906 |
Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-93-9-0906 Last checked: 09/11/2009 Requires: PDF Reader http://apsjournals.apsnet.org/doi/pdfplus/10.1094/PDIS-93-9-0906 Last checked: 09/11/2009 Requires: PDF Reader |
Publication Type:
| Refereed |
Author(s): | Chen, Chi-Min;
de la Cerda, Karla A.;
Kaminski, John E.;
Douhan, Greg W.;
Wong, Francis P. |
Author Affiliation: | Chen, de la Cerda, Douhan and Wong: Department of Plant Pathology, University of California, Riverside, California; Kaminski: Department of Plant Science, University of Connecticut, Storrs, Connecticut |
Title: | Geographic distribution and rDNA-ITS region sequence diversity of Waitea circinata var. circinata isolated from annual bluegrass in the United States |
Section: | Research Other records with the "Research" Section
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Source: | Plant Disease. Vol. 93, No. 9, September 2009, p. 906-911. |
Publishing Information: | St. Paul, MN: American Phytopathological Society |
# of Pages: | 6 |
Keywords: | TIC Keywords: Brown ring patch; DNA; Geographical distribution; Morphology; Poa annua; Waitea circinata var. circinata
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Abstract/Contents: | "Waitea circinata var. circinata is the causal agent of brown ring patch, an emergent disease of turfgrass in the United States. Forty-two isolates from annual bluegrass were obtained from California, Connecticut, Idaho, Illinois, Massachusetts, New York, Ohio, Oregon, and Rhode Island. Almost all isolates produced white to orange sclerotia (bulbils), 2 to 5 mm in size, that turned dark brown after 21 days on ¼-strength potato dextrose agar. The ribosomal DNA internal transcribed spacer regions and 5.8S region (ITS) were analyzed by restriction fragment length polymorphism (RFLP) analysis using MspI and sequencing to attempt identification of the isolates. Some isolates were heterozygous at the mspI restriction site, results not found in previous reports using the RFLP technique for identification. Four additional nucleotide positions were found to be variable within ITS based on sequence analysis, including two indels and two additional heterozygous positions. A total of 17 ITS haplotypes were found, and there was no obvious relationship between ITS haplotype and the geographic distribution of the isolates. Results of this work indicate that W. circinata var. circinata is present in multiple states and provide an initial understanding of the diversity of the pathogen in the United States." |
Language: | English |
References: | 23 |
Note: | Pictures, color Tables |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Chen, C.-M., K. A. de la Cerda, J. E. Kaminski, G. W. Douhan, and F. P. Wong. 2009. Geographic distribution and rDNA-ITS region sequence diversity of Waitea circinata var. circinata isolated from annual bluegrass in the United States. Plant Dis. 93(9):p. 906-911. |
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| DOI: 10.1094/PDIS-93-9-0906 |
| Web URL(s): http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-93-9-0906 Last checked: 09/11/2009 Requires: PDF Reader http://apsjournals.apsnet.org/doi/pdfplus/10.1094/PDIS-93-9-0906 Last checked: 09/11/2009 Requires: PDF Reader |
| MSU catalog number: b2194824a |
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