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| DOI: | 10.1094/PDIS-94-2-0207 |
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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-94-2-0207
Last checked: 03/11/2010
Requires: PDF Reader |
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| Publication Type:
| Refereed |
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| Author(s): | Young, Joseph R.;
Tomaso-Peterson, Maria;
de la Cerda, Karla;
Wong, Francis P. |
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| Author Affiliation: | Young and Tomaso-Peterson: Department of Entomology and Plant Pathology, Mississippi State University, Mississippi State; de la Cerda and Wong: Department of Plant Pathology, Univesity of California, Riverside |
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| Title: | Two mutations in β-tubulin 2 gene associated with thiophanate-methyl resistance in Colletotrichum cereale isolates from creeping bentgrass in Mississippi and Alabama |
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| Source: | Plant Disease. Vol. 94, No. 2, February 2010, p. 207-212. |
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| Publishing Information: | St. Paul, MN: American Phytopathological Society |
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| # of Pages: | 6 |
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| Keywords: | TIC Keywords: Agrostis stolonifera; Colletotrichum graminicola; Fungicide resistance; Genetics; Golf greens; Mutations; Thiophanate-methyl
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| Abstract/Contents: | "Turfgrass anthracnose, caused by Colletotrichum cereale (ΞC. graminicola), has become a common disease of creeping bentgrass putting greens during the summer in Mississippi and Alabama over the last 15 years. Thiophanate-methyl is a single-site mode-of-action fungicide applied to control C. cereale. In vitro bioassays were performed to evaluate the sensitivity of 103 isolates to thiophanate-methyl concentrations ranging from 0.039 to 10 μg/ml. Eighty-three isolates were collected from creeping bentgrass in Mississippi and Alabama that had been exposed to thiophanate-methyl. An additional 20 isolates were included from nonexposed turfgrasses. Radial colony growth in amended media was relative to nonamended media for all in vitro bioassays. With thiophanate-methyl at 10 μg/ml, relative growth of exposed isolates ranged from 77.5 to 130.7% with a mean of 99.3% compared with nonexposed, baseline isolates that ranged from 0.0 to 48.7% with a mean of 20.4%. A representative sample of thiophanate-methyl-exposed and nonexposed isolates was used to determine the mechanism of resistance by comparing amino acid sequences of the β-tubulin 2 protein. All of the thiophanate-methyl-exposed isolates that were sequenced had a point mutation resulting in substitutions from glutamic acid to alanine at position 198 or from phenylalanine to tyrosine at position 200 of the β-tubulin 2 protein. These amino acid substitutions in C. cereale isolates from Mississippi and Alabama appear to confer resistance to thiophanate-methyl and differ from those reported previously for this pathogen." |
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| Language: | English |
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| References: | 37 |
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| Note: | Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Young, J. R., M. Tomaso-Peterson, K. de la Cerda, and F. P. Wong. 2010. Two mutations in β-tubulin 2 gene associated with thiophanate-methyl resistance in Colletotrichum cereale isolates from creeping bentgrass in Mississippi and Alabama. Plant Disease. 94(2):p. 207-212. |
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| DOI: 10.1094/PDIS-94-2-0207 |
| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-94-2-0207
Last checked: 03/11/2010
Requires: PDF Reader |
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MSU catalog number: b2194824a |
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