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| DOI: | 10.2135/cropsci2011.03.0151 |
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| Web URL(s): | https://acsess.onlinelibrary.wiley.com/doi/10.2135/cropsci2011.03.0151
Last checked: 04/05/2024
Access conditions: Item is within a limited-access website
https://acsess.onlinelibrary.wiley.com/doi/epdf/10.2135/cropsci2011.03.0151
Last checked: 04/05/2024
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Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Harris-Shultz, Karen R.;
Milla-Lewis, Susana R.;
Zuleta, M. Carolina;
Schwartz, Brian M.;
Hanna, Wayne W.;
Brady, Jeff A. |
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| Author Affiliation: | Harris-Shultz: Crop Genetics and Breeding Research Unit, USDA-ARS, Tifton, GA; Milla-Lewis and Zuleta: Dep. of Crop Science, North Carolina State University, Raleigh, NC; Schwartz and Hanna: Dep. of Crop & Soil Sciences, The University of Georgia, Tifton, GA; Brady: Texas AgriLife Research, Stephenville, TX |
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| Title: | Development of simple sequence repeat markers and the analysis of genetic diversity and ploidy level in a centipedegrass collection |
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| Section: | Turfgrass science
Other records with the "Turfgrass science" Section
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| Source: | Crop Science. Vol. 52, No. 1, January 2012, p. 383-392. |
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| Publishing Information: | Madison, WI: Crop Science Society of America |
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| # of Pages: | 10 |
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| Keywords: | TIC Keywords: Eremochloa ophiuroides; Eremochloa zeylanica; Genetic variability; Ploidy; Polymerase chain reaction; Simple sequence repeats
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| Abstract/Contents: | "Little is known about the genetic variability of centipedegrass [Eremochloa ophiuroides (Munro) Hack.] and few genetic tools have been available for this species. In this study, 69 unique Eremochloa sequences were generated by using a compound simple sequence repeat (SSR)-based cloning method. Twenty-nine of these clones contained an internal SSR and 30 specific primer pairs were developed that produced suitable amplification. The level of genetic diversity was assessed using 55 centipedegrass accessions and one Eremochloa zeylanica Hack. accession using primer pairs developed from the compound SSR-based cloning technique. Twenty-four polymorphic fragments could be scored and unweighted pair-group method using arithmetic averages (UPGMA) cluster analysis showed that the Eremochloa accessions clustered into two groups: a large cluster of E. ophiuroides accessions and a group containing the single E. zeylanica accession. Principle coordinate analysis further divided the centipedegrass accessions into three groups. Ploidy analysis revealed all centipedegrass accessions were diploid while the single E. zeylanica accession was found to be a putative tetraploid. Furthermore, many of these markers can be used for other species belonging to the subfamily Panicoideae. The division of the centipedegrass accessions into groups and analysis of ploidy level provides information that will aid in the effective use of this germplasm in breeding programs." |
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| Language: | English |
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| References: | 39 |
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| Note: | Figures
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Harris-Shultz, K. R., S. R. Milla-Lewis, M. C. Zuleta, B. M. Schwartz, W. W. Hanna, and J. A. Brady. 2012. Development of simple sequence repeat markers and the analysis of genetic diversity and ploidy level in a centipedegrass collection. Crop Sci. 52(1):p. 383-392. |
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| DOI: 10.2135/cropsci2011.03.0151 |
| Web URL(s):
https://acsess.onlinelibrary.wiley.com/doi/10.2135/cropsci2011.03.0151
Last checked: 04/05/2024
Access conditions: Item is within a limited-access website
https://acsess.onlinelibrary.wiley.com/doi/epdf/10.2135/cropsci2011.03.0151
Last checked: 04/05/2024
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: b2211522a |
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