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| Web URL(s): | https://www.jstor.org/stable/23433377?seq=1#page_scan_tab_contents
Last checked: 06/06/2017
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http://web.archive.org/web/20081205025043/http://www.aosaseed.com/2008_meeting/2008_research.htm
Last checked: 10/01/2014
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Barker, Reed E.;
Davidson, Sharon |
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| Author Affiliation: | Barker: Grass Genomic Testin, Inc.; Davidson: Agri Seed Testing, Inc., Salem, OR |
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| Title: | Allelic discrimination as an aid in determining genetic purity in ryegrass |
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| Meeting Info.: | St. Paul, Minnesota: June 5-12, 2008 |
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| Source: | Seed Technology. Vol. 30, No. 1, 2008, p. 88. |
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| Publishing Information: | Lincoln, Nebraska: Association of Official Seed Analysts and Society of Commercial Seed Technologists |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Alleles; Contamination; Cultivar purity; Genetic analysis; Growth analysis; Lolium
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| Abstract/Contents: | "The seedling root fluorescence (SRF) test has been used to distinguish perennial (Lolium perenne L.) and annual (or Italian) (L. multiflorum) ryegrass since the 1930s. At times the test has been unreliable and overestimates the amount of annual contamination. The objective of our research for the past several years has been to find and characterize specific genes that may be associated with growth type. We have identified alleles (alternate forms of a gene) of three genes associated with flowering control in grasses. Alleles from two of the genes were effective in predicting growth type. Leaf tissue was harvested from seedlings used in an SRF test and DNA extracted using commercially available purification kits. To cut down on lab costs, only seedlings with SRF, plus five to ten seedlings with non-SRF were analyzed on a real-time PCR machine in Allelic Discrimination (AD) mode. Twenty cultivars were tested in a proof-of-concept panel. Following the SRF test, all seedlings were transplanted to a high intensity growth chamber under continuous light for a grow-out test (GOT) that lasted for 84da. Plants reached heading throughout the full time of the GOT, but approached a plateau at about 70da. These results supported that the GOT should be longer than the suggested 42da in order to be effective. Further, SRF was high in the earliest heading plants and declined in later heading plants, but never fell below 30% of the plants heading in each 7da increment demonstrating the problems that the SRF test has in predicting contamination. In contrast, however, AD using alleles from the two genes detected growth type differences to about a 3% level, a level equivalent to a 70da or greater GOT. Detection error rates for the non-SRF plants was less than 0.5% based on presence of two out of three markers that included SRF and alleles of the two genes we used in the study. Allelic Discrimination at the single nucleotide level based on alleles of the Vrn-1 and ID1 genes are an effective and rapid method to predict growth type contamination in ryegrass." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only!
"Abstracts from oral and poster presentations given at the 98th Association of Official Seed Analysts and the 85th Societ of Commercial Seed Technologists (AOSA/SCST) annual meeting" |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Barker, R. E., and S. Davidson. 2008. Allelic discrimination as an aid in determining genetic purity in ryegrass. Seed Technology. 30(1):p. 88. |
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| Web URL(s):
https://www.jstor.org/stable/23433377?seq=1#page_scan_tab_contents
Last checked: 06/06/2017
Requires: Adobe Flash
http://web.archive.org/web/20081205025043/http://www.aosaseed.com/2008_meeting/2008_research.htm
Last checked: 10/01/2014
Notes: Item is within a single large file |
| 
MSU catalog number: b3628131 |
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