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| Publication Type:
| Report |
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| Author(s): | Haygood, R. A.;
Walter, J. F. |
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| Author Affiliation: | Department of Plant Pathology and Physiology, Clemson University, SC |
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| Title: | Biocontrol of brown patch of centipedegrass with Gliocladium virens in a growth chamber, 1989 |
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| Source: | Biological and Cultural Tests for Control of Plant. Vol. 5, 1990, p. 86. |
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| Publishing Information: | St. Paul, MN: The American Phytopathological Society |
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| Keywords: | TIC Keywords: Eremochloa ophiuroides; Brown patch; Rhizoctonia solani
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| Abstract/Contents: | A Cecil sandy loam soil was amended with fertilizer per soil test recommendations and the pH was adjusted to 6.0. The soil was placed in 10-cm-square pots and planted with centipedegrass seed. Plants were maintained at a height of 3 to 4 cm following normal horticultural practices for 6 to 9 months. Inoculum was prepared by growing an isolate of Rhizoctonia solani AG2-2 on a sterile tall-fescue-seed medium (30 g of seed and 31 ml of distilled water) in 500-ml Erlenmeyer flasks. The medium was infested by introducing three 2-cm diameter plugs of 48-hr-old mycelium. The flasks were incubated for 2 weeks at 28 C and shaken daily. Plants were watered well and than inoculated with 1 g of infested-seed inoculum around the sheaths. Sterile fescue seed was placed around crowns of control plants. A dried, bran-alginate prill formulation of Gliocladium virens was introduced around the sheaths 9 days prior to, or at the time of, inoculation. Prills were appled at rates of 0.3 to 10.0 g/pot in three experiments. Pots were covered with clear plastic bags and placed at random in a growth chamber with a 14-hr light, 10-hr dark regime. Temperatures ranged from 28-31 C and 21-23 C in the light and dark regimes, respectively. Six cool white, 115V flourescent tubes and eight 40-W incandescent bulbs provided the light. Ratings based on the percent of sheaths exhibiting symptoms of degradation (rot) were made 10 days after inoculation with the infested seed inoculum. A completely randomized design with four replications per treatment was used in all experiments. The application of a G. virens prill formulation gave significant control of brown patch in all experiments when applied at a rate of at least .8 g/10 cm pot. Excellent control was obtained when the prill was applied at rates of 4,7 and 10 g/pot. No significant differences in control were detected among these rates. Introducing the G. virens prill formulation around sheaths at the time of innoculation with R. solani provided the same degree of control as when it was applied 9 days prior to inoculation." |
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| Language: | English |
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| References: | 0 |
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| See Also: | Other items relating to: CENT |
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| Note: | Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Haygood, R. A., and J. F. Walter. 1990. Biocontrol of brown patch of centipedegrass with Gliocladium virens in a growth chamber, 1989. Biol. Cult. Tests Control Plant Dis. 5:p. 86. |
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