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| DOI: | 10.1007/s00122-009-1142-y |
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| Web URL(s): | https://link.springer.com/article/10.1007%2Fs00122-009-1142-y
Last checked: 10/05/2017 |
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| Publication Type:
| Refereed |
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| Author(s): | Sandhu, Sukhpreet;
James, Victoria A.;
Quesenberry, Kenneth H.;
Altpeter, Fredy |
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| Author Affiliation: | Plant Molecular and Cellular Biology Program, Agronomy Department, Genetics Institute, IFAS, University of Florida, Gainesville, FL |
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| Title: | Risk assessment of transgenic apomictic tetraploid bahiagrass, cytogenetics, breeding behavior and performance of intra-specific hybrids |
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| Source: | TAG: Theoretical and Applied Genetics. Vol. 119, No. 8, November 2009, p. 1383-1395. |
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| Publishing Information: | Berlin: Springer |
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| # of Pages: | 13 |
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| Related Web URL: | http://www.springerlink.com/content/f6817268710015h7/
Last checked: 03/20/2012
Notes: Abstract only |
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| Keywords: | TIC Keywords: Apomixis; Chromatography; Cultivar evaluation; Dry weight; Genetic transformation; Herbicide resistance; Low maintenance; Paspalum notatum; Paspalum simplex; Perennial grasses; Pollination; Restriction fragment length polymorphism; Seed production; Seed setting; Seedheads; Southern blot analysis
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| Cultivar Names: | Argentine; Pensacola; Tifton 7; Tifton 9 |
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| Abstract/Contents: | "Pollen-mediated gene transfer from stress tolerant or herbicide-resistant transgenic plants may cause environmental or agronomic problems. Apomictic seed production found in some bahiagrass cultivars may serve as a natural transgene containment system. Under greenhouse conditions, the average gene transfer frequency from an herbicide-resistant apomictic tetraploid to a population of sexual diploid bahiagrass genotypes or apomictic tetraploid bahiagrass was 0.16% when the transgenic pollen donor was placed at 0.5-1.5 m distance from the non-transgenic pollen receptors. The herbicide-resistant hybrids were characterized for transgene integration, expression and ploidy, by Southern blot analysis, immuno-chromatography and flow cytometry, respectively. Hybrids resulting from open pollination of non-transgenic diploid female plants with transgenic tetraploid male plants were triploids or near-triploids, with 2n = 26-34. These hybrids displayed a wide range of phenotypic variability, including some non-persistent or non-flowering dwarf-type hybrids with good vigor, or hybrids with vegetative growth similar to non-transgenic plants, but with significantly reduced seed set. Non-flowering aneu-triploids with good vigor/field performance will provide the highest level of transgene containment. Embryo sac analysis of pollinated spikelets confirmed a high proportion of aborted ovules. An apospory-linked RFLP marker was detected in 13 of the 15 near-triploid hybrids. All flowering aneuploid hybrids displayed significantly reduced seed set, and none of the sexual near-triploid hybrids produced any seeds. All tetraploid gene transfer events carried the apospory-linked RFLP marker, suggesting that despite the presence of the aposporus locus, a low degree of sexuality co-exists in apomictic tetraploid cultivars. Thus, tetraploid apomictic bahiagrass does not provide complete transgene containment, although intra-specific gene transfer is drastically reduced compared to sexually reproducing perennial grasses." |
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| Language: | English |
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| References: | 74 |
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| Note: | Pictures, color
Figures
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Sandhu, S., V. A. James, K. H. Quesenberry, and F. Altpeter. 2009. Risk assessment of transgenic apomictic tetraploid bahiagrass, cytogenetics, breeding behavior and performance of intra-specific hybrids. Theor. Appl. Genet. 119(8):p. 1383-1395. |
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| DOI: 10.1007/s00122-009-1142-y |
| Web URL(s):
https://link.springer.com/article/10.1007%2Fs00122-009-1142-y
Last checked: 10/05/2017 |
| 
MSU catalog number: b2203373 |
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