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| DOI: | 10.1094/PHYTO-102-7-S4.1 |
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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-102-7-S4.1#page=100
Last checked: 08/10/2012
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Rioux, R.;
van Ryzin, B.;
Kerns, J. |
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| Author Affiliation: | University of Wisconsin-Madison, Madison, WI |
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| Title: | Development of a semiselective media for enhanced detection of Sclerotinia homoeocarpa from plant tissues |
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| Meeting Info.: | Providence, Rhode Island: August 4-8, 2012 |
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| Source: | Phytopathology. Vol. 102, No. 7S, July 2012, p. S4.100. |
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| Publishing Information: | St. Paul, Minnesota: American Phytopathological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Disease control; Disease evaluation; Disease identification; Dollar spot; Growing media; Sclerotinia homoeocarpa
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| Abstract/Contents: | "Dollar spot, caused by the fungus Sclerotinia homoeocarpa is one of the most prevalent diseases on turfgrass worldwide. Due to difficulties identifying sources of inoculum, a more sensitive detection method for S. homoeocarpa is necessary. An improved semi-selective medium for isolation of S. homoeocarpa was developed by comparing growth inhibition on media with various amendments to the current standard, PDA+++ (potato dextrose agar with 50mg/L Tetracycline, Chloramphenicol, and Streptomycin). Amendments included various fungicides, lactic acid, and bromophenol blue for indication of fungal oxalic acid production. Seven-day old agar plugs of four S. homoeocarpa isolates and eight field contaminants were placed in the center of a 60mm diameter petri dish containing PDA with combinations of different amendments. Colony diameter was measured at 48 hours after transfer and percent inhibition was calculated relative to PDA+++. Validation of media that inhibit contaminant growth with minimal impact on S. homoeocarpa was performed by comparing isolation frequency from field and seed samples to PDA+++. Results demonstrate that azoxystrobin is a good amendment for semi-selective media while PCNB is poor because it significantly inhibits S. homoeocarpa growth. Adding bromophenol blue is unnecessary because the color change indicative of acid production is minimal and does not develop fast enough to aid in detection of S. homoeocarpa." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Rioux, R., B. van Ryzin, and J. Kerns. 2012. Development of a semiselective media for enhanced detection of Sclerotinia homoeocarpa from plant tissues. Phytopathology. 102(7S):p. S4.100. |
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| DOI: 10.1094/PHYTO-102-7-S4.1 |
| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-102-7-S4.1#page=100
Last checked: 08/10/2012
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: b2219736a |
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Digitally in TIC by file name: phytp2012julpres |
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