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| DOI: | 10.1093/aob/mcs044 |
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| Web URL(s): | https://academic.oup.com/aob/article/110/6/1327/110559/New-chloroplast-microsatellite-markers-suitable
Last checked: 03/02/2017
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Diekmann, Kerstin;
Hodkinson, Trevor R.;
Barth, Susanne |
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| Author Affiliation: | Diekmann: Teagasc Crops Environment and Land Use Programme, Oak Park Research Centre, Carlow and School of Natural Sciences, Trinity College Dublin, Ireland; Hodkinson: School of Natural Sciences and Trinity Centre for Biodiversity Research, Trinity College Dublin, Ireland; Barth: Teagasc Crops Environment and Land Use Programme, Oak Park Research Centre, Carlow, Ireland |
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| Title: | New chloroplast microsatellite markers suitable for assessing genetic diversity of Lolium perenne and other related grass species |
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| Section: | Breeding strategies for forage and grass improvement
Other records with the "Breeding strategies for forage and grass improvement" Section
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| Source: | Annals of Botany. Vol. 110, No. 6, November 2012, p. 1327-1339. |
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| Publishing Information: | London, England, United Kingdom: Oxford University Press |
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| # of Pages: | 13 |
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| Keywords: | TIC Keywords: Cultivar evaluation; Forage; Genetic variability; Lolium perenne; Microsatellites; Temperate zones
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| Abstract/Contents: | "Background and Aims Lolium perenne (perennial ryegrass) is the most important forage grass species of temperate regions. We have previously released the chloroplast genome sequence of L. perenne 'Cashel'. Here nine chloroplast microsatellite markers are published, which were designed based on knowledge about genetically variable regions within the L. perenne chloroplast genome. These markers were successfully used for characterizing the genetic diversity in Lolium and different grass species. Methods Chloroplast genomes of 14 Poaceae taxa were screened for mononucleotide microsatellite repeat regions and primers designed for their amplification from nine loci. The potential of these markers to assess genetic diversity was evaluated on a set of 16 Irish and 15 European L. perenne ecotypes, nine L. perenne cultivars, other Lolium taxa and other grass species. Key Results All analysed Poaceae chloroplast genomes contained more than 200 mononucleotide repeats (chloroplast simple sequence repeats, cpSSRs) of at least 7 bp in length, concentrated mainly in the large single copy region of the genome. Nucleotide composition varied considerably among subfamilies (with Pooideae biased towards poly A repeats). The nine new markers distinguish L. perenne from all non-Lolium taxa. TeaCpSSR28 was able to distinguish between all Lolium species and Lolium multiflorum due to an elongation of an A89 mononucleotide repeat in L. multiflorum. TeaCpSSR31 detected a considerable degree of microsatellite length variation and single nucleotide polymorphism. TeaCpSSR27 revealed variation within some L. perenne accessions due to a 44-bp indel and was hence readily detected by simple agarose gel electrophoresis. Smaller insertion/deletion events or single nucleotide polymorphisms detected by these new markers could be visualized by polyacrylamide gel electrophoresis or DNA sequencing, respectively. Conclusions The new markers are a valuable tool for plant breeding companies, seed testing agencies and the wider scientific community due to their ability to monitor genetic diversity within breeding pools, to trace maternal inheritance and to distinguish closely related species." |
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| Language: | English |
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| References: | 53 |
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| Note: | Pictures, b/w
Figures
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Diekmann, K., T. R. Hodkinson, and S. Barth. 2012. New chloroplast microsatellite markers suitable for assessing genetic diversity of Lolium perenne and other related grass species. Ann. Bot. 110(6):p. 1327-1339. |
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| DOI: 10.1093/aob/mcs044 |
| Web URL(s):
https://academic.oup.com/aob/article/110/6/1327/110559/New-chloroplast-microsatellite-markers-suitable
Last checked: 03/02/2017
Access conditions: Item is within a limited-access website |
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MSU catalog number: b2209341a |
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