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| Web URL(s): | http://www.nrcresearchpress.com/doi/pdf/10.1139/b92-065
Last checked: 09/29/2015
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Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | van Rooijen, Gijs J. H.;
Wilen, Ronald W.;
Holbrook, Larry A.;
Moloney, Maurice M. |
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| Author Affiliation: | Dept. of Biological Sciences, Univ. of Calgary; Plant Biotechnology Inst., National Research Council of Canada. |
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| Title: | Regulation of accumulation of mRNAs encoding a 20-kDa oil-body protein in microspore-derived embryos of Brassica napus |
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| Source: | Canadian Journal of Botany. Vol. 70, No. 3, March 1992, p. 503-508. |
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| Publishing Information: | Vancouver, British Columbia: The National Research Council of Canada. |
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| Related Web URL: | http://www.nrcresearchpress.com/doi/abs/10.1139/b92-065
Last checked: 09/29/2015
Notes: English abstract only |
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| Keywords: | TIC Keywords: Brassica
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| Abstract/Contents: | "A 990-bp DNA fragment (OB990) encoding part of a 20-kDa Brassica napus oleosin was used to investigate the expression of this gene in microspore-derived embryos. Southern blot analysis of total genomic B. napus DNA indicated that the oleosins comprise a multigene family in B. napus. Northern analysis indicated that oleosin mRNAs are seed-specific and accumulate in zygotic and microspore-derived embryos. Microspore-derived embryos were used to study the hormonal and osmotic regulation of this oleosin gene family. Oleosin mRNAs can be found as early as heart stage. The accumulation of these mRNAs increased dramatically after the application of 10 μM abscistic acid or high osmoticum (12.5% sorbitol). Time course experiments to study the induction of olesosin expression by 10μM abcisic acid and 12.5% sorbitol demonstrated that the abscisic acid and sorbitol effects occur concurrently. This is distinct from the accumulation of napin storage protein mRNAs after the same treatments. When 10μM jasmonic acid was applied, the oseosin mRNA accumulation increases significantly. The possibility that jasmonic acid and abscistic acid may function as intermediaries of the imposed osmotic stress is discussed." and 12.5% sorbitol demonstrated |
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| Language: | English |
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| References: | 32 |
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| Note: | Abstract also appears in French
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
van Rooijen, G. J. H., R. W. Wilen, L. A. Holbrook, and M. M. Moloney. 1992. Regulation of accumulation of mRNAs encoding a 20-kDa oil-body protein in microspore-derived embryos of Brassica napus. Can. J. Bot. 70(3):p. 503-508. |
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| Web URL(s):
http://www.nrcresearchpress.com/doi/pdf/10.1139/b92-065
Last checked: 09/29/2015
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: QK 1 .C3 |
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