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Web URL(s): | http://www.apsnet.org/publications/phytopathology/backissues/Documents/1991Articles/phyto81n10_1133.pdf#page=6 Last checked: 10/15/2015 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Klotz, M. G.;
Hutcheson, S. W. |
Author Affiliation: | University of Maryland, Department of Botany, College Park |
Title: | Characterization of periplasmic catalase isozymes from Pseudomonas syringe pv. glycinea |
Source: | Phytopathology. Vol. 81, No. 10, October 1991, p. 1139. |
Publishing Information: | St. Paul, MN: American Phytopathological Society |
Keywords: | TIC Keywords: Pseudomonas syringae; Isoenzymes; Catalase
|
Abstract/Contents: | "A burst of active oxygen is observed in plant tissue during the initial interaction of Pseudomonas syringae strains with susceptible and resistant hosts which may limit successful colonization. Pathogenic P. syringae strains appear to be able to enzymically modulate active oxygen levels. Since the major active oxygen species contributing to the burst is HOOH, we are studying the role of bacterial catalase in P. syringae pathogenesis. Lysates of pathogenic P. syringae strains contained at least 10-fold higher total catalase activity than lysates of saprophytic bacteria (P. fluorescens, P. putida), independent of their respective growth stages. During early log-phase of growth in KB broth 30% of total catalase activity was detected in periplasmic fluids. By using native PAGE and column chromatography, we have identified 8 apparent isozymes with catalatic activity in lysates of P. s. pv. glycinea. Four isozymes of different size, charge, and kinetic parameters (25 |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Klotz, M. G., and S. W. Hutcheson. 1991. Characterization of periplasmic catalase isozymes from Pseudomonas syringe pv. glycinea. Phytopathology. 81(10):p. 1139. |
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| Web URL(s): http://www.apsnet.org/publications/phytopathology/backissues/Documents/1991Articles/phyto81n10_1133.pdf#page=6 Last checked: 10/15/2015 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: SB 599 .P48 |
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