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| DOI: | 10.1017/S0953756205003370 |
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| Web URL(s): | http://www.sciencedirect.com/science/article/pii/S095375620860204X
Last checked: 11/18/2016
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Glynn, Neil C.;
Hare, Martin C.;
Parry, David W.;
Edwards, Simon G. |
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| Author Affiliation: | Glynn, Hare, Parry and Edwards: Harper Adams University College, Newport, Shropshire; Parry: Loose, Maidstone, Kent, UK; Glynn: USDA-ARS, Sugarcane Field Station, Canal Point, FL |
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| Title: | Phylogenetic analysis of EF-1 alpha gene sequences from isolates of Microdochium nivale leads to elevation of varieties majus and nivale to species status |
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| Source: | Mycological Research. Vol. 109, No. 8, August 2005, p. 872-880. |
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| Publishing Information: | [Amsterdam]: Elsevier |
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| # of Pages: | 9 |
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| Keywords: | TIC Keywords: Microdochium nivale; Phylogenetic analysis; Species identification
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| Abstract/Contents: | "Degenerate PCR primers were designed based on EF-1 alpha (EF-l α) gene sequences of several filamentous fungi retrieved from sequence databases. These primers were used to isolate a partial sequence, approximately 830 bp in length of the EF-l α from isolates of Microdochium nivale obtained from various geographic locations across the world. Two distinct groups of isolates were evident among those isolates examined. Sequence homology for comparisons within group was 99.7 % for group A and 99.8 % for group B. Primers specific to either group A or group B sequences were designed and tested on isolates from around the world. Comparisons were made with primers previously reported for the two varieties of M. nivale and revealed that Group A type isolates correlated with M. nivale var. majus and group B isolates with M. nivale var. nivale. The primers from this study and those previously reported were in agreement for all isolates with the exception of one isolate (NRRL 3289) which failed to amplify with previously published M. nivale primers. Sequence analysis of NRRL 3289 suggested that it was an isolate of M. nivale var. nival as indicated by the EF-l α based primers developed in this study. This study provides sequence based phylogenetic evidence of two species and when taken together with biological differences reported, leads to the recognition of M. majus comb. nov. (syn. Fusarium nivale var. majus). Descriptions of the two species are provided." |
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| Language: | English |
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| References: | 0 |
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| Note: | Tables
Figures |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Glynn, N. C., M. C. Hare, D. W. Parry, and S. G. Edwards. 2005. Phylogenetic analysis of EF-1 alpha gene sequences from isolates of Microdochium nivale leads to elevation of varieties majus and nivale to species status. Mycological Research. 109(8):p. 872-880. |
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| DOI: 10.1017/S0953756205003370 |
| Web URL(s):
http://www.sciencedirect.com/science/article/pii/S095375620860204X
Last checked: 11/18/2016
Access conditions: Item is within a limited-access website |
| 
MSU catalog number: b7174068 |
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