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Web URL(s): | http://www.apsnet.org/publications/phytopathology/backissues/Documents/1992Articles/Phyto82n02_241.pdf#page=3 Last checked: 10/15/2015 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Hayes, Chris;
Harman, Gary;
Stasz, Thomas;
Woo, Sheri;
Pietro, Antonio Di |
Author Affiliation: | Department of Horticultural Sciences, Cornell University |
Title: | Rapid determination of restriction fragment length polymorhisms (RFLP) within the mitochondrial and nuclear cenomes of fusion progeny in Trichoderma Harzianum |
Source: | Phytopathology. Vol. 82, No. 2, February 1992, p. 243. |
Publishing Information: | St. Paul, MN: American Phytopathological Society |
Keywords: | TIC Keywords: Biological control; Pathogens; Fungicides; Genomes
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Abstract/Contents: | "Certain strains of Trichoderma harzianum have been identified as potential biocontrol agents against a variety of plant pathogenic fungi. Alone, these few strains do not possess the ability to be effective biocontrol agents. By combining the beneficial traits of these strains, a superior biocontrol strain could be formed that would be as efficient as chemical fungicides. Since most of these biocontrol strains are asexual, it would be difficult to combine beneficial traits of several strains. Hence, protoplast fusion has been used to combine the nuclei of different strains in an attempt to isolate recombinant strains that contain the biocontrol attributes of both parents. However, during protoplast fusion the mitochondrial genomes, in addition to the nuclear genomes, may also fuse. Monitoring the genetic elements obtained in both genomes has been possible by purifying the genomes to homogeneity on a CsCl density gradient. A more rapid approach has been devised that allows for identification of parental origin of both the mitochondrial and nuclear genomes within a total DNA extract utilizing RFLP." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Hayes, C., G. Harman, T. Stasz, S. Woo, and A. D. Pietro. 1992. Rapid determination of restriction fragment length polymorhisms (RFLP) within the mitochondrial and nuclear cenomes of fusion progeny in Trichoderma Harzianum. Phytopathology. 82(2):p. 243. |
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| Web URL(s): http://www.apsnet.org/publications/phytopathology/backissues/Documents/1992Articles/Phyto82n02_241.pdf#page=3 Last checked: 10/15/2015 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: SB 599 .P48 |
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