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| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/1993/Turfgrass/65325,%20Clemson,%20Baird.PDF
Last checked: 11/19/2013
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Material Type: | Manuscript |
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| Monographic Author(s): | Baird, Wm. Vance; Miller, Landon C. |
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| Author Affiliation: | Baird: P.I.; and Miller: Investigator, Horticulture Department, Clemson University |
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| Monograph Title: | Low Temperature and Drought Regulated Gene Expression in Bermudagrass: [1993 Annual Research Report], 1993. |
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| Publishing Information: | [Clemson, South Carolina]: Horticulture Department, Clemson University |
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| # of Pages: | 3 |
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| Collation: | [3] pp. |
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| Abstract/Contents: | "Nuclear DNA samples have been isolated from three cultivars of bermudagrass ('Midiron', 'Tifway' and 'Tifgreen'). 'U-3' will be included in these studies. The DNA preparations are of sufficient quality (high molecular weight, and free of contaminating proteins and polysaccharides) and quantity that restriction endonuclease digestion can readily be performed. The digested DNA will be used in differential hybridization analysis (Southern blots) of the bermudagrass nuclear genome. Heterologous gene probes for nuclear sequences correlated with induction at low-temperatures or by exposure to conditions of water deficit, originally isolated from dicotyledonous species, will be used to screen these membrane blots. Initial surveys by dot/slot-blot analysis, using undigested genomic DNA and entire plasmid clones as gene probes, indicate that these many of these genes will likely detect related sequences in these cultivars, and these may prove to be evolutionarily conserved in bermudagrass. A postdoctoral research associate (Dr. Jiyu Yan) has recently joined the project, and is focusing here current efforts on this preliminary characterization of the bermudagrass nuclear genome. Institutional support for the purchase of additional growth chambers has been acquired. These chambers will be utilized to reproduce the environmental parameters necessary for low-temperature acclimation. Characterizing the expression of sequences related to the heterologous gene probes under these standardized environmenal conditions is the next objective of this project." |
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| Language: | English |
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| References: | 0 |
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| See Also: | See also related summary article "Low temperature and drought regulated gene expression in bermudagrass", 1993 Turfgrass Research Summary [USGA] 1993, p. 34, R=65325. R=65325 |
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| Note: | Also appears as pp. 00392-00394 in the USGA Turfgrass Research Committee Reporting Binders for 1993.
"Progress Report: May 1 to October 31, 1993"
"C. U. Proposal No: R 0375-67-8461A" |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
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Last checked: 11/19/2013
Requires: PDF Reader |
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