Full TGIF Record # 237595
Item 1 of 1
Publication Type:
i
Report
Author(s):Zheng, Yi-qi; Wang, Zhi-yong; Guo, Hai-lin; Xue, Dan-dan; Liu, Jian-xiu
Author Affiliation:Zheng: Department of Horticulture, Nanjing Agricultural University; Zheng, Wang, Guo, Xue, and Liu: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden Mem. Sun Yat-sen, Nanjing, China
Title:Optimization of SRAP-PCR system on centipedegrass (Eremochloa ophiuroides) using orthogonal design and selection of primers
Source:[Caoye Xuebao] [Acta Prataculturae Sinica]. Vol. 17, No. 4, August 20 2008, p. 110-117.
# of Pages:8
Publishing Information:[Lanzhou Shi, China]: ["Cao Ye Xue Bao" Bian Ji Wei Yuan Hui]
Abstract/Contents:"An orthogonal design was used to optimize a SRAP-PCR system with 4 factors (Mg2+, dNTP, primer and Taq polymerase) at 3 levels plus the concentration of template DNA. The optimized SRAP-PCR system for E. ophiuroides was: 2 μL 10 × PCR buffer, 60 ng template DNA, Mg2+ 1.50 mmol/L, dNTP 260 μmol/L, primer 0.25 μmol/L, Taq DNA polymerase 0.5 U in a total of 20 μL reaction solution. Each factor had a different effect on the results of PCR. Mg2+ concentration had the greatest effect and Taq DNA polymerase had the least effect. The optimized SRAP-PCR system was tested on four E. ophiuroides germplasms and shown to be steady and reliable. 26 primer combinations were selected with abundant polymorphism from 45 primer combinations. The optimized SRAP-PCR system and polymorphism primer combinations could be applied to molecular genetics research of E. ophiuroides."
Language:Chinese
References:44
Note:Abstract also appears in English
"Sum No. 75"
Pictures, b/w
Tables
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Zheng, Y.-q., Z.-y. Wang, H.-l. Guo, D.-d. Xue, and J.-x. Liu. 2008. Optimization of SRAP-PCR system on centipedegrass (Eremochloa ophiuroides) using orthogonal design and selection of primers. (In Chinese) [Caoye Xuebao] [Acta Prataculturae Sinica]. 17(4):p. 110-117.
Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=237595
If there are problems with this record, send us feedback about record 237595.
Choices for finding the above item:
Find Item @ MSU
MSU catalog number: b10289426
Request through your local library's inter-library loan service (bring or send a copy of this TGIF record)