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| Publication Type:
| Report |
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| Author(s): | Zheng, Yi-qi;
Wang, Zhi-yong;
Guo, Hai-lin;
Xue, Dan-dan;
Liu, Jian-xiu |
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| Author Affiliation: | Zheng: Department of Horticulture, Nanjing Agricultural University; Zheng, Wang, Guo, Xue, and Liu: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden Mem. Sun Yat-sen, Nanjing, China |
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| Title: | Optimization of SRAP-PCR system on centipedegrass (Eremochloa ophiuroides) using orthogonal design and selection of primers |
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| Source: | [Caoye Xuebao] [Acta Prataculturae Sinica]. Vol. 17, No. 4, August 20 2008, p. 110-117. |
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| Publishing Information: | [Lanzhou Shi, China]: ["Cao Ye Xue Bao" Bian Ji Wei Yuan Hui] |
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| # of Pages: | 8 |
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| Abstract/Contents: | "An orthogonal design was used to optimize a SRAP-PCR system with 4 factors (Mg2+, dNTP, primer and Taq polymerase) at 3 levels plus the concentration of template DNA. The optimized SRAP-PCR system for E. ophiuroides was: 2 μL 10 × PCR buffer, 60 ng template DNA, Mg2+ 1.50 mmol/L, dNTP 260 μmol/L, primer 0.25 μmol/L, Taq DNA polymerase 0.5 U in a total of 20 μL reaction solution. Each factor had a different effect on the results of PCR. Mg2+ concentration had the greatest effect and Taq DNA polymerase had the least effect. The optimized SRAP-PCR system was tested on four E. ophiuroides germplasms and shown to be steady and reliable. 26 primer combinations were selected with abundant polymorphism from 45 primer combinations. The optimized SRAP-PCR system and polymorphism primer combinations could be applied to molecular genetics research of E. ophiuroides." |
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| Language: | Chinese |
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| References: | 44 |
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| Note: | Abstract also appears in English
"Sum No. 75"
Pictures, b/w
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Zheng, Y.-q., Z.-y. Wang, H.-l. Guo, D.-d. Xue, and J.-x. Liu. 2008. Optimization of SRAP-PCR system on centipedegrass (Eremochloa ophiuroides) using orthogonal design and selection of primers. (In Chinese) [Caoye Xuebao] [Acta Prataculturae Sinica]. 17(4):p. 110-117. |
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