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| DOI: | 10.1016/j.jip.2013.03.006 |
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| Web URL(s): | http://ac.els-cdn.com/S0022201113000384/1-s2.0-S0022201113000384-main.pdf?_tid=6b780c64-aa96-11e6-8b31-00000aacb35e&acdnat=1479147550_6228f53171ee561c22503baf69ef5d56
Last checked: 11/14/2016
Requires: PDF Reader
Access conditions: Item is within a limited-access website
http://www.sciencedirect.com/science/article/pii/S0022201113000384
Last checked: 06/03/2014
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Yamaguchi, Takuya;
Bando, Hisanori;
Asano, Shin-ichiro |
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| Author Affiliation: | Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan |
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| Title: | Identification of a Bacillus thuringiensis Cry8Da toxin-binding glucosidase from the adult Japanese beetle, Popillia japonica |
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| Source: | Journal of Invertebrate Pathology. Vol. 113, No. 2, June 2013, p. 123-128. |
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| Publishing Information: | San Diego: Academic Press |
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| # of Pages: | 6 |
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| Keywords: | TIC Keywords: Bacillus thuringiensis; Nutrient management; Physiological processes; Popillia japonica; Proteins; Toxins
Dutch Turfgrass Research Foundation Keywords: 502-E-2; 500-K; Plant competition; Sward renovation; Festuca; Plant strategies; Golf courses; Article
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| Abstract/Contents: | "Cry8Da from Bacillus thuringiensis galleriae SDS-502 has insecticidal activity against both the larvae and adult Japanese beetle (Popillia japonica Newman). The receptor determines the specificity of the insecticidal activity of Cry proteins and hence, in order to reveal the mode of action of Cry toxin, receptor identification is a necessary step. However, a receptor for Cry8-type toxin has not been identified in the Scarabaeidae family of insects. Therefore, we aimed to identify the receptor of Cry8Da toxin in adult P. japonica BBMV. A ligand blot showed the Cry8Da toxin only bound to a 150 kDa protein in the BBMV of adult P. japonica. In order to identify the Cry8Da toxin binding protein, it was purified by column chromatography and three internal amino acid sequences were determined. Two of the three internal amino acid sequences shared homology with Coleopteran β-glucosidases. In addition, the fraction containing the Cry8Da toxin binding protein had β-glucosidase activity but no aminopeptidase N and alkaline phosphatase activity, both of which are commonly reported as receptors for Cry toxins in Lepidopteran and Dipteran insects. The β-glucosidase homologous genes could be amplified by PCR using degenerate oligonucleotide primers designed from a conserved sequence of Coleopteran β-glucosidases and an internal amino acid sequence of the Cry8Da toxin binding protein. Taken together, the β-glucosidase in adult P. japonica BBMV is the receptor for B. thuringiensis Cry8Da toxin." |
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| Language: | English |
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| References: | 25 |
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| Note: | Pictures, color
Figures
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Yamaguchi, T., H. Bando, and S.-i. Asano. 2013. Identification of a Bacillus thuringiensis Cry8Da toxin-binding glucosidase from the adult Japanese beetle, Popillia japonica. J. Invertebr. Pathol. 113(2):p. 123-128. |
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| DOI: 10.1016/j.jip.2013.03.006 |
| Web URL(s):
http://ac.els-cdn.com/S0022201113000384/1-s2.0-S0022201113000384-main.pdf?_tid=6b780c64-aa96-11e6-8b31-00000aacb35e&acdnat=1479147550_6228f53171ee561c22503baf69ef5d56
Last checked: 11/14/2016
Requires: PDF Reader
Access conditions: Item is within a limited-access website
http://www.sciencedirect.com/science/article/pii/S0022201113000384
Last checked: 06/03/2014
Access conditions: Item is within a limited-access website |
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MSU catalog number: b4889219 |
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