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| DOI: | 10.1080/09583157.2010.491895 |
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| Web URL(s): | http://www.tandfonline.com/doi/full/10.1080/09583157.2010.491895
Last checked: 05/18/2016
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http://www.tandfonline.com/doi/pdf/10.1080/09583157.2010.491895#.Vzyi44QrJpg
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Last checked: 04/22/2016
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| Publication Type:
| Refereed |
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| Author(s): | Pan, L.;
Ash, G. J.;
Ahn, B.;
Watson, A. K. |
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| Author Affiliation: | Pan, Ahn, and Watson: Department of Plant Science, McGill University, Quebec, Canada; Ash: E.H. Graham Centre for Agricultural Innovation, Charles Stuart University, Wagga Wagga, NSW, Australia |
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| Title: | Development of strain specific molecular markers for the Sclerotinia minor bioherbicide strain IMI 344141 |
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| Source: | Biocontrol Science and Technology. Vol. 20, No. 9, 2010, p. 939-959. |
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| Publishing Information: | Abington, Oxfordshire: Carfax Pub. Co. |
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| # of Pages: | 21 |
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| Related Web URL: | http://www.tandfonline.com/doi/abs/10.1080/09583157.2010.491895
Last checked: 04/22/2016
Notes: Abstract only |
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| Keywords: | TIC Keywords: Biological herbicides; Broadleaf weed control; Herbicide evaluation; Molecular biology; Molecular markers; Pathogenic fungi; Polymerase chain reaction; Sclerotinia minor
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| Abstract/Contents: | "The plant pathogenic fungus, Sclerotinia minor IMI 344141, has been developed as a bioherbicide for broadleaf weed control in turfgrass and a means to differentiate this biocontrol agent from like organisms is required. A strain specific molecular marker was developed to detect and monitor the Sclerotinia minor IMI 344141 bioherbicide strain. The method was based on polymerase chain reaction (PCR) amplification of two sequence-characterized amplified regions (SCAR) primer pairs for a first round PCR, and another two sets of nested primers was used for a second round PCR if higher sensitivity was needed. Sclerotinia minor IMI 344141 was successfully traced from both pure cultures and environmental samples originating from bioherbicide-released field trials. DNA of the S. minor bioherbicide isolate IMI 344141 was detected in the soil 2 months after application, but was not detected in the 3- and 9-month samples after application. When applied as a bioherbicide, S. minor (IMI 344141) did not persist into the following spring season in turf environments. This molecular detection method provides a mechanism to distinguish this isolate from related organisms and a tool to monitor behavior of the biocontrol agent S. minor IMI 344141 in nature, particularly in soil." |
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| Language: | English |
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| References: | 31 |
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| Note: | Pictures, b/w
Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Pan, L., G. J. Ash, B. Ahn, and A. K. Watson. 2010. Development of strain specific molecular markers for the Sclerotinia minor bioherbicide strain IMI 344141. Biocontrol Sci. Technol. 20(9):p. 939-959. |
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| DOI: 10.1080/09583157.2010.491895 |
| Web URL(s):
http://www.tandfonline.com/doi/full/10.1080/09583157.2010.491895
Last checked: 05/18/2016
Access conditions: Item is within a limited-access website
http://www.tandfonline.com/doi/pdf/10.1080/09583157.2010.491895#.Vzyi44QrJpg
Last checked: 05/18/2016
Requires: PDF Reader
Access conditions: Item is within a limited-access website
http://www.tandfonline.com/doi/abs/10.1080/09583157.2010.491895#aHR0cDovL3d3dy50YW5kZm9ubGluZS5jb20vZG9pL3BkZi8xMC4xMDgwLzA5NTgzMTU3LjIwMTAuNDkxODk1QEBAMA==
Last checked: 04/22/2016
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: b6434670 |
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